Molecular rotors as reporters for viscosity of solutions of collagen like peptides

Christopher D. McTiernan; Matias Zuñiga-Bustos; Roberto Rosales-Rojas; Pablo Barrias; May Griffith; Horacio Poblete; Peter S. Sherin; Ismael López-Duarte; Marina K. Kuimova; Emilio I. Alarcon

doi:10.1039/D1CP04398F

Molecular rotors as reporters for viscosity of solutions of collagen like peptides†

Christopher D. McTiernan,

^a Matias Zuñiga-Bustos,^b Roberto Rosales-Rojas,^bc Pablo Barrias,

^d May Griffith,

^ef Horacio Poblete,

^bg Peter S. Sherin,

^h Ismael López-Duarte,

^h Marina K. Kuimova

^h and Emilio I. Alarcon

*^ai

Author affiliations

* Corresponding authors

^a Division of Cardiac Surgery, University of Ottawa Heart Institute, 40 Ruskin Street, Ottawa, Canada
E-mail: ealarcon@ottawaheart.ca

^b Departamento de Bioinformática, Centro de Bioinformática, Simulación y Modelado (CBSM), Facultad de Ingeniería, Universidad de Talca, Campus Talca, 1 Poniente No. 1141, Casilla 721, Talca, Chile

^c Doctorado en ciencias Mención Modelado de Sistemas Químicos y Biológicos, Facultad de Ingeniería, Universidad de Talca, Campus Talca, 1 Poniente No. 1141, Casilla 721, Talca, Chile

^d Departamento de Ciencias del Ambiente, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 Correo 33, Santiago, Chile

^e Centre de Recherche Hôpital Maisonneuve-Rosemont, Montréal, QC, Canada

^f Département d'ophtalmologie, Université de Montréal, Montréal, QC, Canada

^g Millennium Nucleus of Ion Channels-Associated Diseases (MiNICAD), Universidad de Talca, Talca, Chile

^h Chemistry Department, Molecular Sciences Research Hub, Imperial College London, 82 Wood Lane, London W12 0BZ, UK

ⁱ Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Canada

Abstract

We have studied the suitability of using a molecular rotor-based steady-state fluorometric assay for evaluating changes in both the conformation and the viscosity of collagen-like peptide solutions. Our results indicate that a positive charge incorporated on the hydrophobic tail of the BODIPY molecular rotor favours the dye specificity as a reporter for viscosity of these solutions.

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Physical Chemistry Chemical Physics

Molecular rotors as reporters for viscosity of solutions of collagen like peptides†

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