Issue 7, 2016

Attenuated total reflection Fourier-transform infrared (ATR-FTIR) imaging of tissues and live cells

Abstract

FTIR spectroscopic imaging is a label-free, non-destructive and chemically specific technique that can be utilised to study a wide range of biomedical applications such as imaging of biopsy tissues, fixed cells and live cells, including cancer cells. In particular, the use of FTIR imaging in attenuated total reflection (ATR) mode has attracted much attention because of the small, but well controlled, depth of penetration and corresponding path length of infrared light into the sample. This has enabled the study of samples containing large amounts of water, as well as achieving an increased spatial resolution provided by the high refractive index of the micro-ATR element. This review is focused on discussing the recent developments in FTIR spectroscopic imaging, particularly in ATR sampling mode, and its applications in the biomedical science field as well as discussing the future opportunities possible as the imaging technology continues to advance.

Graphical abstract: Attenuated total reflection Fourier-transform infrared (ATR-FTIR) imaging of tissues and live cells

Article information

Article type
Review Article
Submitted
30 Jun 2015
First published
21 Okt 2015

Chem. Soc. Rev., 2016,45, 1850-1864

Attenuated total reflection Fourier-transform infrared (ATR-FTIR) imaging of tissues and live cells

K. L. Andrew Chan and S. G. Kazarian, Chem. Soc. Rev., 2016, 45, 1850 DOI: 10.1039/C5CS00515A

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