Issue 1, 2022

Layer-by-layer assembly of multi-layered droplet interface bilayers (multi-DIBs)

Abstract

Droplet interface bilayers (DIBs) have tremendous promise as platforms for fundamental biomembrane studies and in biotechnology. Being composed of a single bilayer however limits their biomimetic potential, as many cell membrane motifs are composed of multiple aligned bilayers. We describe a technology to manufacture cell-sized multi-layered DIBs (multi-DIBs) by coating giant unilamellar vesicles with a further monolayer, and allowing such structures to make contact with themselves or a monolayer coated droplet. This easily customisable strategy will pave the way for an expanded repertoire of DIB functionality, for example by facilitating the incorporation of multiple-bilayer spanning protein complexes.

Graphical abstract: Layer-by-layer assembly of multi-layered droplet interface bilayers (multi-DIBs)

Supplementary files

Article information

Article type
Communication
Submitted
13 Sep 2021
Accepted
30 Nov 2021
First published
01 Dez 2021
This article is Open Access
Creative Commons BY license

Chem. Commun., 2022,58, 60-63

Layer-by-layer assembly of multi-layered droplet interface bilayers (multi-DIBs)

M. E. Allen, J. Albon and Y. Elani, Chem. Commun., 2022, 58, 60 DOI: 10.1039/D1CC05155E

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