Issue 4, 2018

Enhanced cell adhesion on a bio-inspired hierarchically structured polyester modified with gelatin-methacrylate

Abstract

Herein, fabrication and modification of novel bio-inspired microwell arrays with nanoscale topographic structures are reported. The natural nano- and microstructures present on the surface of rose petals were hypothesized to enhance cell-surface contacts. Thus hierarchically structured polyethylene terephthalate glycol modified (PETG) substrates were fabricated by replication from rose petals via nanoimprint lithography, followed by covalent modification and crosslinking with RGD-presenting gelatin-methacrylate (GelMA) for promoting cell adhesion and spreading. Cell culture experiments showed that the introduction of gelatin resulted in significantly enhanced cell adhesion and more than doubled cell areas on the GelMA modified surfaces. In addition, a slight preference was observed for concave compared to convex surfaces, which is tentatively attributed to the matching curvature of the micro-cavities and the cells, facilitating the accommodation of cells. These bioinspired hierarchically structured and gelatin functionalized substrates may provide new prospects for designing cell-based interfaces for advanced biomedical studies, e.g. for cell culture and biosensing in the future.

Graphical abstract: Enhanced cell adhesion on a bio-inspired hierarchically structured polyester modified with gelatin-methacrylate

Supplementary files

Article information

Article type
Paper
Submitted
30 Okt 2017
Accepted
24 Nov 2017
First published
27 Nov 2017

Biomater. Sci., 2018,6, 785-792

Enhanced cell adhesion on a bio-inspired hierarchically structured polyester modified with gelatin-methacrylate

P. Li, X. Dou, C. Feng and H. Schönherr, Biomater. Sci., 2018, 6, 785 DOI: 10.1039/C7BM00991G

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