Issue 1, 2018

Combined determination of copper ions and β-amyloid peptide by a single ratiometric electrochemical biosensor

Abstract

Copper ions (Cu2+) play a critical role in biological processes and are directly involved in β-amyloid peptide (Aβ) aggregation, which is responsible for the occurrence and development of Alzheimer's disease (AD). Therefore, combined determination of Cu2+ and Aβ in one analytical system is of great significance to understand the exact nature of the AD event. This work presents a novel ratiometric electrochemical biosensor for the dual determination of Cu2+ and Aβ1–42. This unique sensor is based on a 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS) and poly(diallyldimethylammonium chloride) (PDDA)-bi functionalized single-walled carbon nanotubes (ABTS-PDDA/CNTs) composite. The inclusion of ABTS not only enhanced the sensitivity, but it also acted as an inner reference molecule to improve detection accuracy. The specific recognition of Cu2+ was realized by neurokinin B (NKB) coatings on the ABTS-PDDA/CNTs surface to form a [CuII(NKB)2] complex with Cu2+. The ABTS-PDDA/CNTs-NKB modified electrode also displayed an excellent electrochemical response toward the Aβ1–42 monomer, when a certain amount of the Aβ1–42 monomer was added to Cu2+-contained PBS buffer, which was due to the release of Cu2+ from the [CuII(NKB)2] complex through Aβ binding to Cu2+. Meanwhile, our work showed that Cu2+ bound Aβ1–42 was concentration-dependent. Consequently, the presented electrochemical approach was capable of quantifying two important biological species associated with AD by one single biosensor, with the detection limits of 0.04 μM for Cu2+ and 0.5 ng mL−1 for Aβ1–42, respectively. Finally, the ratiometric electrode was successfully applied for monitoring Cu2+ and Aβ1–42 variations in plasma and hippocampus of normal and AD rats.

Graphical abstract: Combined determination of copper ions and β-amyloid peptide by a single ratiometric electrochemical biosensor

Supplementary files

Article information

Article type
Paper
Submitted
12 Okt 2017
Accepted
15 Nov 2017
First published
15 Nov 2017

Analyst, 2018,143, 323-331

Combined determination of copper ions and β-amyloid peptide by a single ratiometric electrochemical biosensor

Y. Yu, P. Wang, X. Zhu, Q. Peng, Y. Zhou, T. Yin, Y. Liang and X. Yin, Analyst, 2018, 143, 323 DOI: 10.1039/C7AN01683B

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