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We report the use of fluorescence lifetime measurements for investigating two-photon polymerization. For this purpose, a molecular rotor was successfully exploited as a viscosity probe to reveal heterogeneity in multi-material microstructures made by two-photon polymerization. Furthermore, a correlation between laser intensity and local viscosity changes in the polymerized microstructures was established.

Graphical abstract: Investigation of two-photon polymerized microstructures using fluorescence lifetime measurements

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