Issue 3, 2018

Advances in the synthesis of nitroxide radicals for use in biomolecule spin labelling

Abstract

EPR spectroscopy is an increasingly useful analytical tool to probe biomolecule structure, dynamic behaviour, and interactions. Nitroxide radicals are the most commonly used radical probe in EPR experiments, and many methods have been developed for their synthesis, as well as incorporation into biomolecules using site-directed spin labelling. In this Tutorial Review, we discuss the most practical methods for the synthesis of nitroxides, focusing on the tunability of their structures, the manipulation of their sidechains into spin labelling handles, and their installation into biomolecules.

Graphical abstract: Advances in the synthesis of nitroxide radicals for use in biomolecule spin labelling

Article information

Article type
Tutorial Review
Submitted
20 Jul 2016
First published
01 Dez 2017

Chem. Soc. Rev., 2018,47, 668-680

Advances in the synthesis of nitroxide radicals for use in biomolecule spin labelling

M. M. Haugland, J. E. Lovett and E. A. Anderson, Chem. Soc. Rev., 2018, 47, 668 DOI: 10.1039/C6CS00550K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements