Issue 2, 2023

A multienzyme biocatalytic cascade as a route towards the synthesis of α,ω-diamines from corresponding cycloalkanols

Abstract

A multienzymatic cascade for the synthesis of 1,6-hexamethylenediamine (HMD) and related α,ω-diamines from corresponding cycloalkanols is reported herein. In this work, an E coli-based modularization strategy was applied to distribute the complete biosynthetic pathway into different modules. The engineered transaminase variant SPTA_R417F has a beneficial impact on the cascade by driving the reaction towards target product formation. This new variant has lost >95% activity towards detrimental pyruvate, generated via auxiliary intracellular glucose metabolism in a host cell. The designed E. coli consortium including respective modules has produced 42% of HMD (from 10 mM cyclohexanol) in a one-pot-one-step manner. This developed route is the first example of a biocatalytic synthesis of HMD and related α,ω-diamines from corresponding cycloalkanols by employing E. coli-based consortia.

Graphical abstract: A multienzyme biocatalytic cascade as a route towards the synthesis of α,ω-diamines from corresponding cycloalkanols

Supplementary files

Article information

Article type
Communication
Submitted
08 Sept 2022
Accepted
09 Nov 2022
First published
10 Nov 2022

Green Chem., 2023,25, 543-549

A multienzyme biocatalytic cascade as a route towards the synthesis of α,ω-diamines from corresponding cycloalkanols

S. Sarak, A. D. Pagar, T. P. Khobragade, H. Jeon, P. Giri, S. Lim, M. D. Patil, Y. Kim, B. Kim and H. Yun, Green Chem., 2023, 25, 543 DOI: 10.1039/D2GC03392E

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