Issue 95, 2022

Luminescence detection of peptide:N-glycanase activity using engineered split inteins

Abstract

A split intein-based method has been developed to detect peptide:N-glycanase (PNGase) activity in live cells. PNGase cleaves the linkage between N,N′-diacetylchitobiose and the Asn side-chain of N-intein peptides and the products react rapidly with C-intein by protein trans-splicing to generate an active luciferase.

Graphical abstract: Luminescence detection of peptide:N-glycanase activity using engineered split inteins

Supplementary files

Article information

Article type
Communication
Submitted
02 Sept 2022
Accepted
07 Nov 2022
First published
08 Nov 2022

Chem. Commun., 2022,58, 13282-13285

Luminescence detection of peptide:N-glycanase activity using engineered split inteins

T. Takahashi, T. Uchibayashi, N. Ishii, I. Matsuo, Y. Yoshida and T. Suzuki, Chem. Commun., 2022, 58, 13282 DOI: 10.1039/D2CC04865E

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