Impact of dissolved sulfide on a hybrid membrane bioreactor treating the effluent of a mainstream up-flow anaerobic sludge blanket

Abstract

Despite being toxic to some microbes in wastewater treatment, sulfide can also promote nitrogen removal through sulfide-oxidizing bacteria. This study evaluates the dissolved sulfide impact on a hybrid MBR treating the effluent of a mainstream UASB. A UASB-MBR (176 L) was fed with synthetic domestic sewage and operated for 154 days. Two periods were distinguished, one without (Period I) and one with (Period II) sulfide dissolved in the UASB effluent. Dissolved methane, COD, nitrogen, and organic micropollutants (OMP)s removals accomplished in the MBR during both periods were compared. Initially, sulfide inhibited methane removal, but once fully oxidized into sulfate in the anoxic compartment, the efficiencies recovered to similar levels as without sulfide (>70%). Sulfide additions significantly enhanced the MBR denitrification potential through sulfide-oxidizing bacteria, with improved removals in Period II (63.4 TN L_feed⁻¹) compared to Period I (40 mg TN L_feed⁻¹). Most of the nitrogen removal occurred in the anoxic compartment of the MBR, however, up to 21% of the nitrogen was denitrified in the aerobic compartment within the biofilm carriers. Aerobic methane oxidation coupled with denitrification, heterotrophic denitrifiers, sulfide oxidation, and anammox processes were involved in the nitrogen removal. COD and OMPs removals were not affected by sulfide.