ESIPT-based fluorescence probe for the rapid detection of peroxynitrite ‘AND’ biological thiols

Luling Wu; Hai-Hao Han; Liyuan Liu; Jordan E. Gardiner; Adam C. Sedgwick; Chusen Huang; Steven D. Bull; Xiao-Peng He; Tony D. James

doi:10.1039/C8CC06917D

ESIPT-based fluorescence probe for the rapid detection of peroxynitrite ‘AND’ biological thiols†

Luling Wu,

‡^a Hai-Hao Han,

‡^b Liyuan Liu,

^a Jordan E. Gardiner,

^a Adam C. Sedgwick,

^ac Chusen Huang,

*^d Steven D. Bull,

*^a Xiao-Peng He

*^b and Tony D. James

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, University of Bath, Bath, UK
E-mail: t.d.james@bath.ac.uk, s.d.bull@bath.ac.uk

^b Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai 200237, China
E-mail: xphe@ecust.edu.cn

^c Department of Chemistry, University of Texas at Austin, 105 E 24th Street A5300, Austin, TX 78712-1224, USA

^d The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China
E-mail: huangcs@shnu.edu.cn

Abstract

An ESIPT-based ‘AND’ logic fluorescence probe (GSH-ABAH) was developed for the simultaneous detection of ONOO⁻ and biological thiols. GSH-ABAH was shown to have good cell permeability and with the addition of just SIN-1 (ONOO⁻ donor) or GSH, no fluorescence response was observed in live cells. However, in the presence of both analytes GSH-ABAH could be used to image exogenous ONOO⁻ ‘AND’ GSH added to RAW264.7 cells.

This article is part of the themed collection: The Mechanics of Supramolecular Chemistry

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DOI: https://doi.org/10.1039/C8CC06917D
Article type: Communication
Submitted: 25 Sext 2018
Accepted: 11 Sept 2018
First published: 11 Sept 2018
This article is Open Access

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Chem. Commun., 2018,54, 11336-11339

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ESIPT-based fluorescence probe for the rapid detection of peroxynitrite ‘AND’ biological thiols

L. Wu, H. Han, L. Liu, J. E. Gardiner, A. C. Sedgwick, C. Huang, S. D. Bull, X. He and T. D. James, Chem. Commun., 2018, 54, 11336 DOI: 10.1039/C8CC06917D

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Chemical Communications

ESIPT-based fluorescence probe for the rapid detection of peroxynitrite ‘AND’ biological thiols†

Abstract

Supplementary files

Article information

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ESIPT-based fluorescence probe for the rapid detection of peroxynitrite ‘AND’ biological thiols

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