Syntheses of the first representatives of C-glycosyl s-tetrazines and demonstration of their synthetic utility to get barely known C-glycosyl pyridazines.
We formalized a unique working mechanism – internal conversion to a dark state (ICDS) to rationalize the fluorogenicity of the tetrazine-fused fluorophores with integrated π-conjugations.
A regioselective synthesis of trisubstituted pyridazines from tetrazines and alkynyl sulfides is disclosed. Good transformability of sulfur-substituents allowed us to synthesize various pyridazines.
The effect of substited 3,6-R2-1,2,4,5-tetrazines (R = Me, MeO, H or Cl) is probed in radical-bridged Ln2 metallocenes. Strong magnetic Ln–rad coupling is observed, which leads to slow magnetic relaxation and open hysteresis loops for the Dy analogs.
Light-activated fluorescence represents a potent tool for investigating subcellular structures and dynamics, offering enhanced control over the temporal and spatial aspects of the fluorescence signal.