Photoactivatable synthetic fluorophores
The concatenation of a photochemical transformation with a photophysical process can be exploited to activate the fluorescence of synthetic chromophores under optical control. The resulting photoactivatable fluorophores permit the monitoring of dynamic processes in real time and the reconstruction of images with spatial resolution at the nanometer level.
Activation of BODIPY fluorescence by the photoinduced dealkylation of a pyridinium quencher
The photoinduced transformation of a pyridinium cation into a pyridine ring suppresses electron transfer from an adjacent BODIPY fluorophore upon excitation and activates the emission of the latter.
Determination of two-photon photoactivation rates of fluorescent proteins
We report wavelength-dependent two-photon activation rates of PAmCherry, PAmKate and PA-GFP measured in microscopic sample volumes.
Sensitized excited free-radical processes as read–write tools: impact on non-linear lithographic processes
A dye-tethered TEMPO radical abstracts hydrogen upon photoexcitation enabling erasable fluorescent non-linear imaging on thin polymer films.
Understanding super-resolution nanoscopy and its biological applications in cell imaging
Photoswitchable fluorophores play critical roles in enabling superresolution imaging, thus the key determinant for future superresolution nanoscopy.
Radical diffusion limits to photoinhibited superresolution lithography
Fast diffusion of photo-generated reactive species has thwarted recent attempts at optical patterning below the diffraction limit.
About this collection
The “Superresolution imaging and fabrication with light” themed issue contains a collection of articles on this emerging area of research. The issue is Guest Edited by Françisco M. Raymo (University of Miami, USA).