Issue 42, 2019

Systematic investigation of a quantitative paper-based DNA reader (qPDR) for distance-based quantification of nucleic acids and mercury ions

Abstract

Nucleic acid testing using microfluidic paper-based analytical devices (μPAD) offers exciting opportunities for inexpensive, portable diagnostics in resource-limited settings. We have previously developed a portable, affordable, and easy-to-fabricate quantitative paper-based DNA reader (qPDR) capable of quantifying nucleic acids by measuring distance as a digital readout. Here, to decipher specific molecular interactions and identify optimal experimental conditions for using qPDR, we systematically investigated the chromatographic behaviours of SYBR Green I (SG), a key assay component, in the presence of DNA, organic solvents, saline, urea, and surfactants. We also demonstrated the possibility to expand qPDR for the detection of non-nucleic-acid targets. Specifically, we have successfully enabled distance-based quantification of mercury ions with nanomolar detection limit using thymine-rich DNA and qPDR.

Graphical abstract: Systematic investigation of a quantitative paper-based DNA reader (qPDR) for distance-based quantification of nucleic acids and mercury ions

Article information

Article type
Paper
Submitted
01 جوٗلایی 2019
Accepted
06 اکتوٗبر 2019
First published
07 اکتوٗبر 2019

Anal. Methods, 2019,11, 5376-5380

Systematic investigation of a quantitative paper-based DNA reader (qPDR) for distance-based quantification of nucleic acids and mercury ions

T. Dong, G. A. Wang, M. W. Li and F. Li, Anal. Methods, 2019, 11, 5376 DOI: 10.1039/C9AY01383K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements