Issue 36, 2013

Protein triggered fluorescence switching of near-infrared emitting nanoparticles for contrast-enhanced imaging

Abstract

Sub-100 nm colloidal particles which are surface-functionalized with multiple environmentally-sensitive moieties have the potential to combine imaging, early detection, and the treatment of cancer with a single type of long-circulating “nanodevice”. Deep tissue imaging is achievable through the development of particles which are surface-modified with fluorophores that operate in the near-infrared (NIR) spectrum and where the fluorophore's signal can be maximized by “turning-on” the fluorescence only in the targeted tissue. We present a general approach for the synthesis of NIR emitting nanoparticles that exhibit a protein triggered activation/deactivation of the emission. Dispersing the particles into an aqueous solution, such as phosphate buffered saline (PBS), resulted in an aggregation of the hydrophobic fluorophores and a cessation of emission. The emission can be reinstated, or activated, by the conversion of the surface-attached fluorophores from an aggregate to a monomeric species with the addition of an albumin. This activated probe can be deactivated and returned to a quenched state by a simple tryptic digestion of the albumin. The methodology for emission switching offers a path to maximize the signal from the typically weak quantum yield inherent in NIR fluorophores.

Graphical abstract: Protein triggered fluorescence switching of near-infrared emitting nanoparticles for contrast-enhanced imaging

Supplementary files

Article information

Article type
Paper
Submitted
14 میٔ 2013
Accepted
18 جوٗلایی 2013
First published
18 جوٗلایی 2013

J. Mater. Chem. B, 2013,1, 4542-4554

Protein triggered fluorescence switching of near-infrared emitting nanoparticles for contrast-enhanced imaging

R. Jetty, Y. P. Bandera, M. A. Daniele, D. Hanor, Hsin-I. Hung, V. Ramshesh, M. F. Duperreault, A. Nieminen, J. J. Lemasters and S. H. Foulger, J. Mater. Chem. B, 2013, 1, 4542 DOI: 10.1039/C3TB20681E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements