Issue 15, 2024

A pH-response-based fluorescent probe for detecting the mitophagy process by tracing changes in colocalization coefficients

Abstract

Mitochondria are not only the center of energy metabolism but also involved in regulating cellular activities. Quality and quantity control of mitochondria is therefore essential. Mitophagy is a lysosome-dependent process to clear dysfunctional mitochondria, and abnormal mitophagy can cause metabolic disorders. Therefore, it is necessary to monitor the mitophagy in living cells on a real-time basis. Herein, we developed a pH-responsive fluorescent probe MP for the detection of the mitophagy process using real-time tracing colocalization coefficients. Probe MP showed good pH responses with high selectivity and sensitivity in spectral testing. Probe MP is of positive charge, which is beneficial for accumulating into mitochondrial in living cells. Cells exhibited pH-dependent fluorescence when they were treated with different pH media. Importantly, the changes in the colocalization coefficient between probe MP and Lyso Tracker® Deep Red from 0.4 to 0.8 were achieved in a real-time manner during the mitophagy stimulated by CCCP, starvation and rapamycin. Therefore, combined with the parameter of the colocalization coefficient, probe MP is a potential molecular tool for the real-time tracing of mitophagy to further explore the details of mitophagy.

Graphical abstract: A pH-response-based fluorescent probe for detecting the mitophagy process by tracing changes in colocalization coefficients

Supplementary files

Article information

Article type
Paper
Submitted
03 فرؤری 2024
Accepted
11 مارٕچ 2024
First published
12 مارٕچ 2024

Anal. Methods, 2024,16, 2241-2247

A pH-response-based fluorescent probe for detecting the mitophagy process by tracing changes in colocalization coefficients

N. Hao, Z. Jiang, L. Zhou, X. Dai and X. Kong, Anal. Methods, 2024, 16, 2241 DOI: 10.1039/D4AY00211C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements