Issue 21, 2020

Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules

Abstract

Sensors based on fluorogenic RNA aptamers have emerged in recent years. These sensors have been used for in vitro and intracellular detection of a broad range of biological and medical targets. However, the potential application of fluorogenic RNA-based sensors for point-of-care testing is still little studied. Here, we report a paper substrate-based portable fluorogenic RNA sensor system. Target detection can be simply performed by rehydration of RNA sensor-embedded filter papers. This affordable sensor system can be used for the selective, sensitive, and rapid detection of different target analytes, such as antibiotics and cellular signaling molecules. We believe that these paper-based fluorogenic RNA sensors show great potential for point-of-care testing of a wide range of targets from small molecules, nucleic acids, proteins, to various pathogens.

Graphical abstract: Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules

Supplementary files

Article information

Article type
Paper
Submitted
22 مارٕچ 2020
Accepted
06 میٔ 2020
First published
20 میٔ 2020

Anal. Methods, 2020,12, 2674-2681

Author version available

Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules

F. Shafiei, K. McAuliffe, Y. Bagheri, Z. Sun, Q. Yu, R. Wu and M. You, Anal. Methods, 2020, 12, 2674 DOI: 10.1039/D0AY00588F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements