Issue 7, 2018

Fabrication of gelatin-micropatterned surface and its effect on osteogenic differentiation of hMSCs

Abstract

Studies of the effect of surface chemistries on cell functions on a single surface with different surface chemistry areas have been achieved by micropatterning technology. In this study, a gelatin-micropatterned surface was prepared and used for direct comparison of the effect of different surface chemistries on the osteogenic differentiation of stem cells under the same culture environment. The micropattern was prepared by UV-irradiating photo-reactive azidobenzoyl-derived gelatin-coated surface on a polystyrene plate through a photomask with 200 μm-width stripes. Human bone marrow-derived mesenchymal stem cells (hMSCs) were cultured on the gelatin-modified or micropatterned surfaces in osteogenic differentiation induction medium. Alkaline phosphatase (ALP) staining, alizarin red S staining and real-time PCR analysis were performed to detect the effect of surface chemistry on the osteogenic differentiation of hMSCs. The cells on both the polystyrene area and the gelatin-micropatterned area showed positive ALP staining. Alizarin red S staining results showed that the gelatin-micropatterned area promoted the deposition of minerals. Real-time PCR results indicated that the gelatin-modified surface promoted the expression of osteogenic specific genes encoding ALP and bone sialoprotein. The results suggested the promotive effect of gelatin on the osteogenic differentiation of hMSCs.

Graphical abstract: Fabrication of gelatin-micropatterned surface and its effect on osteogenic differentiation of hMSCs

Article information

Article type
Paper
Submitted
08 دسمبر 2017
Accepted
20 جنؤری 2018
First published
22 جنؤری 2018

J. Mater. Chem. B, 2018,6, 1018-1025

Fabrication of gelatin-micropatterned surface and its effect on osteogenic differentiation of hMSCs

L. Guo, Y. Fan, N. Kawazoe, H. Fan, X. Zhang and G. Chen, J. Mater. Chem. B, 2018, 6, 1018 DOI: 10.1039/C7TB03165C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements