Tucker J. Carrocci, Lea Lohe, Matthew J. Ashton, Claudia Höbartner and Aaron A. Hoskins
Chem. Commun., 2017,53, 11992-11995
DOI:
10.1039/C7CC06703H,
Communication
The 10DM24 deoxyribozyme can site-specifically label RNAs with fluorophore-GTP conjugates; however, the 2′,5′-branched RNA linkage is readily cleaved by debranchase. To prevent loss of labels upon cleavage, we synthesized phosphorothioate-modified, fluorescent GTP derivatives and elaborated conditions for their incorporation by 10DM24. RNAs labeled with fluorescent derivatives of Sp-GTPS were found to be resistant to debranchase.
