A ratiometric fluorescent sensor for the mitochondrial copper pool

Clara Shen; Jacek L. Kolanowski; Carmen M.-N. Tran; Amandeep Kaur; Mia C. Akerfeldt; Matthew S. Rahme; Trevor W. Hambley; Elizabeth J. New

doi:10.1039/C6MT00083E

A ratiometric fluorescent sensor for the mitochondrial copper pool†

Clara Shen,^a Jacek L. Kolanowski,^a Carmen M.-N. Tran,^a Amandeep Kaur,^a Mia C. Akerfeldt,^a Matthew S. Rahme,^a Trevor W. Hambley^a and Elizabeth J. New

*^a

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^a School of Chemistry, The University of Sydney, NSW 2006, Australia
E-mail: Elizabeth.new@sydney.edu.au

Abstract

Copper plays a key role in the modulation of cellular function, defence, and growth. Here we present InCCu1, a ratiometric fluorescent sensor for mitochondrial copper, which changes from red to blue emission in the presence of Cu(I). Employing this probe in microscopy and flow cytometry, we show that cisplatin-treated cells have an impaired ability to accumulate copper in the mitochondria.

This article is part of the themed collection: Mammalian Copper Transport and Related Disorders

Metallomics

A ratiometric fluorescent sensor for the mitochondrial copper pool†

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