Issue 7, 2022

Landscaping macrocyclic peptides: stapling hDM2-binding peptides for helicity, protein affinity, proteolytic stability and cell uptake

Abstract

Cyclic peptides that modulate protein–protein interactions can be valuable therapeutic candidates if they can be delivered intact to their target proteins in cells. Here we systematically compare the effects of different helix-inducing cyclization constraints on the capacity of a macrocyclic peptide component to confer α-helicity, protein-binding affinity, resistance to degradative proteases and cell uptake to a 12-residue peptide fragment of tumor suppressor protein p53. We varied the helix-inducing constraint (hydrocarbon, lactam, aliphatic or aromatic thioether, etc.) and the position of the cyclization linker (i to i + 4 or i to i + 7 bridges) in order to sculpt the macrocyclic size, stabilize its structure, and promote cell uptake. We find that rigidifying the macrocycle leads to higher alpha helicity, target affinity and proteolytic stability to different extents, whereas cell uptake of compounds shown here is mostly driven by hydrophobicity and aromaticity of the macrocycle.

Graphical abstract: Landscaping macrocyclic peptides: stapling hDM2-binding peptides for helicity, protein affinity, proteolytic stability and cell uptake

Supplementary files

Article information

Article type
Paper
Submitted
01 Dec 2021
Accepted
19 May 2022
First published
31 May 2022
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2022,3, 895-904

Landscaping macrocyclic peptides: stapling hDM2-binding peptides for helicity, protein affinity, proteolytic stability and cell uptake

A. D. de Araujo, J. Lim, K. Wu, H. N. Hoang, H. T. Nguyen and D. P. Fairlie, RSC Chem. Biol., 2022, 3, 895 DOI: 10.1039/D1CB00231G

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