Issue 84, 2013
From the journal:

Chemical Communications

Sandwich assay for mixed-sequence recognition of double-stranded DNA: Invader-based detection of targets specific to foodborne pathogens†

Benjamin Denn,a   Saswata Karmakar,a   Dale C. Guenthera  and  Patrick J. Hrdlicka*a  

* Corresponding authors

a Department of Chemistry, University of Idaho, 875 Perimeter Drive MS 2343, Moscow, ID 83844-2343, USA
E-mail: hrdlicka@uidaho.edu
Fax: +1 208 885 6173
Tel: +1 208 885 0108

Abstract

A 96-well plate sandwich assay based on Invader capture/signalling probes is used to recognize 28-mer mixed-sequence dsDNA targets specific to Salmonella enterica, Campylobacter jejuni, Escherichia coli. Targets are detected down to 20–55 pM concentration with excellent binding specificity.

Graphical abstract: Sandwich assay for mixed-sequence recognition of double-stranded DNA: Invader-based detection of targets specific to foodborne pathogens

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Article information

DOI
https://doi.org/10.1039/C3CC45705B
Article type
Communication
Submitted
26 Jul 2013
Accepted
09 Sep 2013
First published
09 Sep 2013

Chem. Commun., 2013,49, 9851-9853

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Sandwich assay for mixed-sequence recognition of double-stranded DNA: Invader-based detection of targets specific to foodborne pathogens

B. Denn, S. Karmakar, D. C. Guenther and P. J. Hrdlicka, Chem. Commun., 2013, 49, 9851 DOI: 10.1039/C3CC45705B

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