In this review, recent progress in tetrazine-based fluorogenic probes for bioorthogonal chemistry is comprehensively analyzed, with a focus on advancements in probe design and their imaging applications for molecular processes in live cells.
Peptides were radiolabeled with [18F]AmBF3-Tz using IEDDA-based tetrazine ligation, producing radiopeptides in different oxidation states. In this study, the formed tautomers were rapidly (10 min) converted to the stable oxidized radiopeptides.
This review highlights the mechanisms underlying the unique fluorogenic properties of tetrazine-functionalized labels and proposes new strategies to enhance fluorogenicity in the near-infrared region.
A new radiotracer [68Ga]Ga-THP-tetrazine for bioorthogonal click radiochemistry is presented. Its ability for pretargeted imaging of long-circulating liposomal nanomedicines was tested in vitro and in vivo in both healthy and tumour murine models.
The incorporation of tetrazine bioorthogonal chemistry with nanoparticles has greatly expanded the biological applications of nanotechnologies.