The missing pieces in the catalytic cycle of [FeFe] hydrogenases

Manon T. Lachmann, Zehui Duan, Patricia Rodríguez-Maciá and James A. Birrell

Chem. Sci., 2024, 15, 14062
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Investigating the role of the strong field ligands in [FeFe] hydrogenase: spectroscopic and functional characterization of a semi-synthetic mono-cyanide active site

Marco Lorenzi, Joe Gellett, Afridi Zamader, Moritz Senger, Zehui Duan, Patricia Rodríguez-Maciá and Gustav Berggren

Chem. Sci., 2022, 13, 11058
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Photochemistry and photoinhibition of the H-cluster of FeFe hydrogenases

Matteo Sensi, Carole Baffert, Vincent Fourmond, Luca de Gioia, Luca Bertini and Christophe Léger

Sustainable Energy Fuels, 2021, 5, 4248
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Redox tuning of the H-cluster by second coordination sphere amino acids in the sensory [FeFe] hydrogenase from Thermotoga maritima

Nipa Chongdar, Patricia Rodríguez-Maciá, Edward J. Reijerse, Wolfgang Lubitz, Hideaki Ogata and James A. Birrell

Chem. Sci., 2023, 14, 3682
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Secondary structure changes as the potential H2 sensing mechanism of group D [FeFe]-hydrogenases

Ivan Voloshyn, Conrad Schumann, Princess R. Cabotaje, Afridi Zamader, Henrik Land and Moritz Senger

Chem. Commun., 2024, 60, 10914
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Indications for a universal hydrogen catalysis mechanism in [FeFe]-hydrogenases of different phylogenetic groups

Moritz Senger, Conrad Schumann, Princess R. Cabotaje, Afridi Zamader, Ping Huang, Henrik Land and Gustav Berggren

Phys. Chem. Chem. Phys., 2025, 27, 9864
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Hydride state accumulation in native [FeFe]-hydrogenase with the physiological reductant H2 supports its catalytic relevance

Moritz Senger, Tobias Kernmayr, Marco Lorenzi, Holly J. Redman and Gustav Berggren

Chem. Commun., 2022, 58, 7184
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Oxygen sensitivity of [FeFe]-hydrogenase: a comparative study of active site mimics inside vs. outside the enzyme

Shanika Yadav, Rieke Haas, Esma Birsen Boydas, Michael Roemelt, Thomas Happe, Ulf-Peter Apfel and Sven T. Stripp

Phys. Chem. Chem. Phys., 2024, 26, 19105
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Outer-sphere effects on the O2 sensitivity, catalytic bias and catalytic reversibility of hydrogenases

Andrea Fasano, Vincent Fourmond and Christophe Léger

Chem. Sci., 2024, 15, 5418
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The isomer-sensitive electrochemical HER of ruthenium(ii)–hydrido complexes involving redox-active azoheteroaromatics

Liton Seikh, Suman Dhara, Ajit Kumar Singh, Aditi Singh, Sanchaita Dey, Arindam Indra and Goutam Kumar Lahiri

Dalton Trans., 2024, 53, 1746
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E. coli-based semi-artificial photosynthesis: biocompatibility of redox mediators and electron donors in [FeFe] hydrogenase driven hydrogen evolution

Mira T. Gamache, Larissa Kurth, Dawit T. Filmon, Nicolas Plumeré and Gustav Berggren

Energy Adv., 2023, 2, 2085
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Evidence of Atypical Structural Flexibility of the Active Site Surrounding of an [FeFe] Hydrogenase from Clostridium beijerinkii

Patrick S. Corrigan, Sean H. Majer and Alexey Silakov

J. Am. Chem. Soc., 2023, 145, 11033
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Unimolecular and Bimolecular Pathways in Bidirectional Redox Molecular Catalysis

Vincent Fourmond, Antoine Jacob-Villedieu, A. Jalila Simaan, Bruno Faure and Christophe Léger

J. Am. Chem. Soc., 2025, 147, 35788
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Evaluation of Biohydrogen Production Depending on the Substrate Used—Examples for the Development of Green Energy

Zbigniew Jarosz, Magdalena Kapłan, Kamila Klimek, Dorota Anders, Barbara Dybek, Marcin Herkowiak, Jakub T. Hołaj-Krzak, Serhiy Syrotyuk, Serhiy Korobka, Hanna Syrotyuk and Grzegorz Wałowski

Energies, 2024, 17, 2524
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Reconsidering the Enzyme Kinetics of [FeFe]-Hydrogenases: Improved Turnover Rates and New Insights into pH and Potential Dependence with Eu(II)-Based Solution Assays

Eda Sönmez, Nikolaos Kostopoulos, Mira Gamache, Mun Hon Cheah, Ping Huang, Andrew J. Bagnall, Dawit T. Filmon, Ivan Voloshyn, Thomas Happe, Moritz Senger, Nicolas Plumeré, Alina Sekretareva and Gustav Berggren

Anal. Chem., 2025, 97, 26393
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In Situ Infrared Spectroscopy for the Analysis of Gas-processing Metalloenzymes

Sven T. Stripp

ACS Catal., 2021, 11, 7845
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Catalytic Bias and Redox-Driven Inactivation of the Group B FeFe Hydrogenase CpIII

Andrea Fasano, Aurore Jacq-Bailly, Jeremy Wozniak, Vincent Fourmond and Christophe Léger

ACS Catal., 2024, 14, 7001
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Reversible or Irreversible Catalysis of H+/H2 Conversion by FeFe Hydrogenases

Andrea Fasano, Henrik Land, Vincent Fourmond, Gustav Berggren and Christophe Léger

J. Am. Chem. Soc., 2021, 143, 20320
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Semi-synthetic hydrogenases—in vitro and in vivo applications

Lívia S. Mészáros, Henrik Land, Holly J. Redman and Gustav Berggren

Current Opinion in Green and Sustainable Chemistry, 2021, 32, 100521
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The catalytic cycle of [FeFe] hydrogenase: A tale of two sites

James A. Birrell, Patricia Rodríguez-Maciá, Edward J. Reijerse, Maria Alessandra Martini and Wolfgang Lubitz

Coordination Chemistry Reviews, 2021, 449, 214191
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Photosynthetic hydrogen production: Novel protocols, promising engineering approaches and application of semi‐synthetic hydrogenases

Sergey Kosourov, Maximilian Böhm, Moritz Senger, Gustav Berggren, Karin Stensjö, Fikret Mamedov, Peter Lindblad and Yagut Allahverdiyeva

Physiologia Plantarum, 2021, 173, 555
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Second and Outer Coordination Sphere Effects in Nitrogenase, Hydrogenase, Formate Dehydrogenase, and CO Dehydrogenase

Sven T. Stripp, Benjamin R. Duffus, Vincent Fourmond, Christophe Léger, Silke Leimkühler, Shun Hirota, Yilin Hu, Andrew Jasniewski, Hideaki Ogata and Markus W. Ribbe

Chem. Rev., 2022, 122, 11900
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Fantastic [FeFe]-Hydrogenases and Where to Find Them

Simone Morra

Front. Microbiol., 2022, 13
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Kinetic Modeling of the Reversible or Irreversible Electrochemical Responses of FeFe-Hydrogenases

Andrea Fasano, Carole Baffert, Conrad Schumann, Gustav Berggren, James A. Birrell, Vincent Fourmond and Christophe Léger

J. Am. Chem. Soc., 2024, 146, 1455
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Phonon-assisted electron-proton transfer in [FeFe] hydrogenases: Topological role of clusters

Yann Chalopin, Stephen P. Cramer and Simon Arragain

Biophysical Journal, 2023, 122, 1557
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Probing the Influence of the Protein Scaffold on H-Cluster Reactivity via Gain-of-Function Studies─Improved H2 Evolution and O2 Tolerance through Rational Design of [FeFe] Hydrogenase

Princess R. Cabotaje, Alina Sekretareva, Moritz Senger, Ping Huang, Kaija Walter, Holly J. Redman, Nicholas Croy, Sven T. Stripp, Henrik Land and Gustav Berggren

J. Am. Chem. Soc., 2025, 147, 4654
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Insights into the Molecular Mechanism of Formaldehyde Inhibition of [FeFe]-Hydrogenases

Jifu Duan, Astrit Veliju, Oliver Lampret, Lingling Liu, Shanika Yadav, Ulf-Peter Apfel, Fraser A. Armstrong, Anja Hemschemeier and Eckhard Hofmann

J. Am. Chem. Soc., 2023, 145, 26068
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A Bidirectional Bioinspired [FeFe]-Hydrogenase Model

Md Estak Ahmed, Abhijit Nayek, Alenka Križan, Nathan Coutard, Adina Morozan, Somdatta Ghosh Dey, Reiner Lomoth, Leif Hammarström, Vincent Artero and Abhishek Dey

J. Am. Chem. Soc., 2022, 144, 3614
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Protein Dynamics Affect O2-Stability of Group B [FeFe]-Hydrogenase from Thermosediminibacter oceani

Subhasri Ghosh, Chandan K. Das, Sarmila Uddin, Sven T. Stripp, Vera Engelbrecht, Martin Winkler, Silke Leimkühler, Claudia Brocks, Jifu Duan, Lars V. Schäfer and Thomas Happe

J. Am. Chem. Soc., 2025, 147, 15170
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Proton reduction catalyzed by monodentate and chelating phosphine-substituted diiron complexes bridged by 4-hydroxythiophenol: Functional [FeFe] H2ase models

Ritu and Sandeep Kaur-Ghumaan

Journal of Molecular Structure, 2026, 1353, 144398
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Probing Substrate Transport Effects on Enzymatic Hydrogen Catalysis: An Alternative Proton Transfer Pathway in Putatively Sensory [FeFe] Hydrogenase

Princess R. Cabotaje, Kaija Walter, Afridi Zamader, Ping Huang, Felix Ho, Henrik Land, Moritz Senger and Gustav Berggren

ACS Catal., 2023, 13, 10435
DOI: 10.1021/acscatal.3c02314

Minimal and hybrid hydrogenases are active from archaea

Chris Greening, Princess R. Cabotaje, Luis E. Valentin Alvarado, Pok Man Leung, Henrik Land, Thiago Rodrigues-Oliveira, Rafael I. Ponce-Toledo, Moritz Senger, Max A. Klamke, Michael Milton, Rachael Lappan, Susan Mullen, Jacob West-Roberts, Jie Mao, Jiangning Song, Marie Schoelmerich, Courtney W. Stairs, Christa Schleper, Rhys Grinter, Anja Spang, Jillian F. Banfield and Gustav Berggren

Cell, 2024, 187, 3357
DOI: 10.1016/j.cell.2024.05.032

Semisynthetic [FeFe]-hydrogenase with stable expression and H2 production capacity in a photosynthetic microbe

Adam Wegelius, Henrik Land, Gustav Berggren and Peter Lindblad

Cell Reports Physical Science, 2021, 2, 100376
DOI: 10.1016/j.xcrp.2021.100376

Improving sustainable hydrogen production from green waste: [FeFe]-hydrogenases quantitative gene expression RT-qPCR analysis in presence of autochthonous consortia

M. Arizzi, S. Morra, G. Gilardi, M. Pugliese, M. L. Gullino and F. Valetti

Biotechnol Biofuels, 2021, 14
DOI: 10.1186/s13068-021-02028-3

Mechanism of Hydrogen Sulfide-Dependent Inhibition of FeFe Hydrogenase

Christina Felbek, Federica Arrigoni, David de Sancho, Aurore Jacq-Bailly, Robert B. Best, Vincent Fourmond, Luca Bertini and Christophe Léger

ACS Catal., 2021, 11, 15162
DOI: 10.1021/acscatal.1c04838

Homogeneous electrocatalytic water splitting by main group element porphyrins

Ni Wang, Yaochang Wang, Xin Liu, Ju Liu, Chenxiao Guo, Xing Deng, Jiangwei Liang, Minglei Cao and Ni Wang

International Journal of Hydrogen Energy, 2025, 177, 151412
DOI: 10.1016/j.ijhydene.2025.151412

A personal account on 25 years of scientific literature on [FeFe]-hydrogenase

Jason W. Sidabras and Sven T. Stripp

J Biol Inorg Chem, 2023, 28, 355
DOI: 10.1007/s00775-023-01992-5

Reversible catalysis

Vincent Fourmond, Nicolas Plumeré and Christophe Léger

Nat Rev Chem, 2021, 5, 348
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A [FeFe] Hydrogenase–Rubrerythrin Chimeric Enzyme Functions to Couple H2 Oxidation to Reduction of H2O2 in the Foodborne Pathogen Clostridium perfringens

Jesse Taylor, David W. Mulder, Patrick S. Corrigan, Michael W. Ratzloff, Natalia Irizarry Gonzalez, Carolyn E. Lubner, Paul W. King and Alexey Silakov

J. Am. Chem. Soc., 2025, 147, 9764
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Proton Transfer Mechanisms in Bimetallic Hydrogenases

Hulin Tai, Shun Hirota and Sven T. Stripp

Acc. Chem. Res., 2021, 54, 232
DOI: 10.1021/acs.accounts.0c00651

H-cluster Intermediates and Catalytic Properties of Clostridium pasteurianum [FeFe]-Hydrogenase III

Effie C. Kisgeropoulos, Michael W. Ratzloff, Ekaterina M. Stroeva-Dahl, Sarah Hasan, Febin Varghese, Jacob H. Artz, John W. Peters, David W. Mulder and Paul W. King

Biochemistry, 2025, 64, 2455
DOI: 10.1021/acs.biochem.5c00066

Developing high-affinity, oxygen-insensitive [NiFe]-hydrogenases as biocatalysts for energy conversion

Chris Greening, Ashleigh Kropp, Kylie Vincent and Rhys Grinter

Biochemical Society Transactions, 2023, 51, 1921
DOI: 10.1042/BST20230120