Highly specific and sensitive detection of microRNAs by tandem signal amplification based on duplex-specific nuclease and strand displacement

Qing Liu, Peng-Jian Kang, Zeng-Ping Chen, Cai-Xia Shi, Yao Chen and Ru-Qin Yu

Chem. Commun., 2019, 55, 14210
DOI: 10.1039/C9CC06790F

Multiplex miRNA assay using lanthanide-tagged probes and the duplex-specific nuclease amplification strategy

Shixi Zhang, Rui Liu, Zhi Xing, Sichun Zhang and Xinrong Zhang

Chem. Commun., 2016, 52, 14310
DOI: 10.1039/C6CC08334J

Optical nano-biosensing interface via nucleic acid amplification strategy: construction and application

Hong Zhou, Jing Liu, Jing-Juan Xu, Shu-Sheng Zhang and Hong-Yuan Chen

Chem. Soc. Rev., 2018, 47, 1996
DOI: 10.1039/C7CS00573C

Ultrasensitive detection of microRNAs using catalytic hairpin assembly coupled with enzymatic repairing amplification

Chong-Hua Zhang, Ying Tang, Ying-Ying Sheng, Hui Wang, Zhan Wu and Jian-Hui Jiang

Chem. Commun., 2016, 52, 13584
DOI: 10.1039/C6CC07116C

Universal and label-free photosensitization colorimetric assays enabled by target-induced termini transformation of dsDNA resistant to Exo III digestion

Xianming Li, Lu Gao, Feng Li, Xiandeng Hou and Peng Wu

Chem. Commun., 2019, 55, 7211
DOI: 10.1039/C9CC03551F

Ultrasensitive photoelectrochemical biosensor for MiRNA-21 assay based on target-catalyzed hairpin assembly coupled with distance-controllable multiple signal amplification

Ming-Hui Zhu, Xiao-Mei Mu, Han-Mei Deng, Xia Zhong, Ruo Yuan and Ya-Li Yuan

Chem. Commun., 2019, 55, 9622
DOI: 10.1039/C9CC04987H

A dual signal amplification strategy for the highly sensitive fluorescence detection of nucleic acids

Jingjing Zhang, Chunyuan Song, Huiling Zhou, Juan Jia, Yinna Dai, Daxiang Cui, Lianhui Wang and Lixing Weng

Analyst, 2020, 145, 1219
DOI: 10.1039/C9AN02183C

In situ generation of electrochemiluminescent DNA nanoflowers as a signal tag for mucin 1 detection based on a strategy of target and mimic target synchronous cycling amplification

Sheng-Kai Li, An-Yi Chen, Xiao-Xue Niu, Zhi-Ting Liu, Min Du, Ya-Qin Chai, Ruo Yuan and Ying Zhuo

Chem. Commun., 2017, 53, 9624
DOI: 10.1039/C7CC04747A

Quantum material accompanied nonenzymatic cascade amplification for ultrasensitive photoelectrochemical DNA sensing

Guangming Wen, Xiaojie Yang and Yongzhao Wang

J. Mater. Chem. B, 2017, 5, 7775
DOI: 10.1039/C7TB01846K

Sensitive detection of intracellular microRNA based on a flowerlike vector with catalytic hairpin assembly

Jintong Liu, Ping Du, Jing Zhang, Hong Shen and Jianping Lei

Chem. Commun., 2018, 54, 2550
DOI: 10.1039/C7CC09579A

Dual catalytic DNA circuit-induced gold nanoparticle aggregation: An enzyme-free and colorimetric strategy for amplified detection of nucleic acids

Hadi Ravan, Mojdeh Amandadi, Mehdi Hassanshahian and Shahram Pourseyedi

International Journal of Biological Macromolecules, 2020, 154, 896
DOI: 10.1016/j.ijbiomac.2020.03.059

Hairpin Assembly Amplified Electrochemical Biosensor for Highly Sensitive and Specific Detection of DNA

Changli Zhong, Gang Yang, Nian Wang, Feihu Ji, Ye Zhang, Xiaojuan Ding, Junhong Yang and Jian Zhang

Electroanalysis, 2016, 28, 1578
DOI: 10.1002/elan.201501178

A POCT assay based on commercial HCG strip for miRNA21 detection by integrating with RCA-HCR cascade amplification and CRISPR/Cas12a

Yuan Li, Zhihui Zeng, Xuefei Lv, Hao Jiang, Anyi Li, Ying Liu, Yulin Deng and Xiaoqiong Li

Microchim Acta, 2025, 192
DOI: 10.1007/s00604-024-06922-z

Recent advances in duplex-specific nuclease-based signal amplification strategies for microRNA detection

Yudong Wu, Shuang Cui, Qian Li, Renshuai Zhang, Zhengming Song, Yuanzhen Gao, Wujun Chen and Dongming Xing

Biosensors and Bioelectronics, 2020, 165, 112449
DOI: 10.1016/j.bios.2020.112449

Electrochemical analysis of microRNAs with hybridization chain reaction-based triple signal amplification

Jianfeng Ma, Lingbo Gong, Yingying Cen, Lin Feng, Yan Su, Xingfen Liu, Jie Chao, Ying Wan, Shao Su and Lianhui Wang

Chinese Chemical Letters, 2023, 34, 108012
DOI: 10.1016/j.cclet.2022.108012

Facile detection of microRNA based on phosphorescence resonance energy transfer and duplex-specific nuclease-assisted signal amplification

Jia-jia Yang, Zhi-feng Zhang and Gui-qin Yan

Analytical Biochemistry, 2017, 539, 127
DOI: 10.1016/j.ab.2017.10.021

Ratiometric electrochemical assay for sensitive detecting microRNA based on dual-amplification mechanism of duplex-specific nuclease and hybridization chain reaction

Yan-Hong Yuan, Bao-Zhu Chi, Shao-Hua Wen, Ru-Ping Liang, Zhi-Mei Li and Jian-Ding Qiu

Biosensors and Bioelectronics, 2018, 102, 211
DOI: 10.1016/j.bios.2017.11.030

Carbon dots hybrid for dual fluorescent detection of microRNA-21 integrated bioimaging of MCF-7 using a microfluidic platform

Somayeh Mohammadi, Abdollah Salimi, Zohreh Hoseinkhani, Foad Ghasemi and Kamran Mansouri

J Nanobiotechnol, 2022, 20
DOI: 10.1186/s12951-022-01274-3

Principles and Applications of Nucleic Acid Strand Displacement Reactions

Friedrich C. Simmel, Bernard Yurke and Hari R. Singh

Chem. Rev., 2019, 119, 6326
DOI: 10.1021/acs.chemrev.8b00580

Applications of Catalytic Hairpin Assembly Reaction in Biosensing

Jumei Liu, Ye Zhang, Huabin Xie, Li Zhao, Lei Zheng and Huiming Ye

Small, 2019, 15
DOI: 10.1002/smll.201902989

Nitrogen-Doped Graphene Quantum Dots@SiO2 Nanoparticles as Electrochemiluminescence and Fluorescence Signal Indicators for Magnetically Controlled Aptasensor with Dual Detection Channels

Chengquan Wang, Jing Qian, Kun Wang, Mengjuan Hua, Qian Liu, Nan Hao, Tianyan You and Xingyi Huang

ACS Appl. Mater. Interfaces, 2015, 7, 26865
DOI: 10.1021/acsami.5b09300

Colorimetric detection of microRNA based hybridization chain reaction for signal amplification and enzyme for visualization

Na Ying, Taifan Sun, Zhibao Chen, Guangping Song, Bingyao Qi, Shengjun Bu, Xiuwei Sun, Jiayu Wan and Zehong Li

Analytical Biochemistry, 2017, 528, 7
DOI: 10.1016/j.ab.2017.04.007

One-to-Many Single Entity Electrochemistry Biosensing for Ultrasensitive Detection of microRNA

Yi-Yan Bai, Zhen Wu, Chun-Miao Xu, Li Zhang, Jiao Feng, Dai-Wen Pang and Zhi-Ling Zhang

Anal. Chem., 2020, 92, 853
DOI: 10.1021/acs.analchem.9b03492

Improving the Signal-to-Background Ratio during Catalytic Hairpin Assembly through Both-End-Blocked DNAzyme

Li Deng, Yuanheng Wu, Shuxia Xu, Yurong Tang, Xinfeng Zhang and Peng Wu

ACS Sens., 2018, 3, 1190
DOI: 10.1021/acssensors.8b00243

Emerging Biosensing Approaches for microRNA Analysis

Richard M. Graybill and Ryan C. Bailey

Anal. Chem., 2016, 88, 431
DOI: 10.1021/acs.analchem.5b04679

A Peptide Cleavage-Based Ultrasensitive Electrochemical Biosensor with an Ingenious Two-Stage DNA Template for Highly Efficient DNA Exponential Amplification

Ding Wang, Yaqin Chai, Yali Yuan and Ruo Yuan

Anal. Chem., 2017, 89, 8951
DOI: 10.1021/acs.analchem.7b01477

Ultrasensitive Photoelectrochemical Biosensor Based on DNA Tetrahedron as Nanocarrier for Efficient Immobilization of CdTe QDs-Methylene Blue as Signal Probe with Near-Zero Background Noise

Mengjie Li, Chuan Xiong, Yingning Zheng, Wenbin Liang, Ruo Yuan and Yaqin Chai

Anal. Chem., 2018, 90, 8211
DOI: 10.1021/acs.analchem.8b01641

A luminescent microRNA nanoprobe based on the target-triggered release of an iridium(III)-solvent complex from mesoporous silica nanoparticles

Xiaomin Tang, Kaimei Fan, Baohan Qu and Lihua Lu

Microchim Acta, 2019, 186
DOI: 10.1007/s00604-019-3895-6

Enzyme-free amplified detection of circulating microRNA by making use of DNA circuits, a DNAzyme, and a catalytic hairpin assembly

Jie Luo, Yongjie Xu, Jian Huang, Shu Zhang, Qi Xu and Jun He

Microchim Acta, 2018, 185
DOI: 10.1007/s00604-017-2565-9

Target-triggered DNA nanoassembly on quantum dots and DNAzyme-modulated double quenching for ultrasensitive microRNA biosensing

Rui Yuan, Xiaolin Yu, Yuhong Zhang, Lulu Xu, Wei Cheng, Zhiguang Tu and Shijia Ding

Biosensors and Bioelectronics, 2017, 92, 342
DOI: 10.1016/j.bios.2016.11.002

[Ru(dcbpy)2dppz]2+/Fullerene Cosensitized PTB7‐Th for Ultrasensitive Photoelectrochemical MicroRNA Assay

Ling‐Ying Xia, Ying‐Ning Zheng, Wen‐Bin Liang, Meng‐Jie Li, Tao Hu, Ruo Yuan and Ya‐Qin Chai

Chemistry A European J, 2019, 25, 4087
DOI: 10.1002/chem.201806005

Sensitive electrochemical biosensor for MicroRNAs based on duplex-specific nuclease-assisted target recycling followed with gold nanoparticles and enzymatic signal amplification

Hui Zhang, Mengxing Fan, Juqian Jiang, Qingming Shen, Chenxin Cai and Jian Shen

Analytica Chimica Acta, 2019, 1064, 33
DOI: 10.1016/j.aca.2019.02.060

Lateral flow nucleic acid biosensor for sensitive detection of microRNAs based on the dual amplification strategy of duplex-specific nuclease and hybridization chain reaction

Na Ying, Chuanjing Ju, Xiuwei Sun, Letian Li, Hongbiao Chang, Guangping Song, Zhongyi Li, Jiayu Wan, Enyong Dai and Pierre Busson

PLoS ONE, 2017, 12, e0185091
DOI: 10.1371/journal.pone.0185091

Colorimetric detection of microRNA based on DNAzyme and nuclease-assisted catalytic hairpin assembly signal amplification

Hongmei Zhang, Kuiyu Wang, Shengjun Bu, Zhongyi Li, Chuanjing Ju and Jiayu Wan

Molecular and Cellular Probes, 2018, 38, 13
DOI: 10.1016/j.mcp.2018.02.002

Electrochemical detection of microRNAs based on AuNPs/CNNS nanocomposite with Duplex-specific nuclease assisted target recycling to improve the sensitivity

Xiangyu Ma, Haoran Xu, Kun Qian, Martha Kandawa-Schulz, Weimin Miao and Yihong Wang

Talanta, 2020, 208, 120441
DOI: 10.1016/j.talanta.2019.120441

Optomagnetic Detection of MicroRNA Based on Duplex-Specific Nuclease-Assisted Target Recycling and Multilayer Core-Satellite Magnetic Superstructures

Bo Tian, Jing Ma, Zhen Qiu, Teresa Zardán Gómez de la Torre, Marco Donolato, Mikkel Fougt Hansen, Peter Svedlindh and Mattias Strömberg

ACS Nano, 2017, 11, 1798
DOI: 10.1021/acsnano.6b07763

Duplex-specific nuclease-based electrochemical biosensor for the detection of microRNAs by conversion of homogeneous assay into surface-tethered electrochemical analysis

Lin Liu, Dehua Deng, Daohong Wu, Weilin Hou, Lu Wang, Ning Li and Zhifang Sun

Analytica Chimica Acta, 2021, 1149, 338199
DOI: 10.1016/j.aca.2021.338199

Chemical Measurement and Analysis: from Phenomenon to Essence

Wei Zhao and Jing‐Juan Xu

Chin. J. Chem., 2022, 40, 1975
DOI: 10.1002/cjoc.202200134

Quantification of MicroRNAs by Coupling Cyclic Enzymatic Amplification with Microfluidic Voltage-Assisted Liquid Desorption Electrospray Ionization Mass Spectrometry

Xiangtang Li, Pratik Rout, Rui Xu, Li Pan, Paul B. Tchounwou, Yonggang Ma and Yi-Ming Liu

Anal. Chem., 2018, 90, 13663
DOI: 10.1021/acs.analchem.8b04008

Metal Ion-Mediated Potential-Resolved Ratiometric Electrochemiluminescence Bioassay for Efficient Determination of miR-133a in Early Diagnosis of Acute Myocardial Infarction

Jue Wang, Mohammad A. Haghighatbin, Wen Shen, Lan Mi and Hua Cui

Anal. Chem., 2020, 92, 7062
DOI: 10.1021/acs.analchem.0c00377

Modified beacon probe assisted dual signal amplification for visual detection of microRNA

Xiuwei Sun, Na Ying, Chuanjing Ju, Zhongyi Li, Na Xu, Guijuan Qu, Wensen Liu and Jiayu Wan

Analytical Biochemistry, 2018, 550, 68
DOI: 10.1016/j.ab.2018.04.010

A novel nonenzymatic cascade amplification for ultrasensitive photoelectrochemical DNA sensing based on target driven to initiate cyclic assembly of hairpins

Guangming Wen, Wenxia Dong, Bin Liu, Zhongping Li and Lifang Fan

Biosensors and Bioelectronics, 2018, 117, 91
DOI: 10.1016/j.bios.2018.05.054

Rapid and ultrasensitive detection of microRNA by target-assisted isothermal exponential amplification coupled with poly (thymine)-templated fluorescent copper nanoparticles

Kwan Woo Park, Bhagwan S Batule, Kyoung Suk Kang, Ki Soo Park and Hyun Gyu Park

Nanotechnology, 2016, 27, 425502
DOI: 10.1088/0957-4484/27/42/425502

Recent advances in catalytic hairpin assembly signal amplification-based sensing strategies for microRNA detection

Yan Wu, Cuicui Fu, Wenbing Shi and Jinyang Chen

Talanta, 2021, 235, 122735
DOI: 10.1016/j.talanta.2021.122735

Universal Ratiometric Photoelectrochemical Bioassay with Target-Nucleotide Transduction-Amplification and Electron-Transfer Tunneling Distance Regulation Strategies for Ultrasensitive Determination of microRNA in Cells

Ying-Ning Zheng, Wen-Bin Liang, Cheng-Yi Xiong, Ying Zhuo, Ya-Qin Chai and Ruo Yuan

Anal. Chem., 2017, 89, 9445
DOI: 10.1021/acs.analchem.7b02270

Multi-Amplified Sensing of MicroRNA by a Small DNA Fragment-Driven Enzymatic Cascade Reaction

Eunjung Kim, Philip D. Howes, Spencer W. Crowder and Molly M. Stevens

ACS Sens., 2017, 2, 111
DOI: 10.1021/acssensors.6b00601

Contrary Logic Pair Library, Parity Generator/Checker and Various Concatenated Logic Circuits Engineered by a Label‐Free and Immobilization‐Free Electrochemiluminescence Resonance Energy Transfer System

Liping Zhu, Linying Yu, Tian Meng, Yao Peng and Xiurong Yang

Small, 2021, 17
DOI: 10.1002/smll.202102881

Duplex-Specific Nuclease-Amplified Detection of MicroRNA Using Compact Quantum Dot–DNA Conjugates

Ye Wang, Philip D. Howes, Eunjung Kim, Christopher D. Spicer, Michael R. Thomas, Yiyang Lin, Spencer W. Crowder, Isaac J. Pence and Molly M. Stevens

ACS Appl. Mater. Interfaces, 2018, 10, 28290
DOI: 10.1021/acsami.8b07250

Label-free visual biosensor based on cascade amplification for the detection of Salmonella

Yuan Zhang, Jingjing Tian, Kai Li, Hongtao Tian and Wentao Xu

Analytica Chimica Acta, 2019, 1075, 144
DOI: 10.1016/j.aca.2019.05.020

A dual-signaling electrochemical ratiometric strategy combining “signal-off” and “signal-on” approaches for detection of MicroRNAs

H. Rezaei, M. Hosseini and S. Radfar

Analytical Biochemistry, 2021, 632, 114356
DOI: 10.1016/j.ab.2021.114356

Construction of a Molybdenum Disulfide-Based Colorimetric Sensor for Label-Free Infectious Disease Analysis Coupled with a Catalyzed Hairpin Assembly Reaction

Yan Dong, Ling Wan, Suo Lv, Dan Zhu, Shao Su, Jie Chao and Lianhui Wang

Langmuir, 2022, 38, 1791
DOI: 10.1021/acs.langmuir.1c02891

RNA‐Cleaving DNAzyme‐Based Amplification Strategies for Biosensing and Therapy

Jiaxin Su, Chenyang Sun, Jinya Du, Xiaotong Xing, Fang Wang and Haifeng Dong

Adv Healthcare Materials, 2023, 12
DOI: 10.1002/adhm.202300367

Application of hairpin DNA-based biosensors with various signal amplification strategies in clinical diagnosis

Rozita Abolhasan, Amir Mehdizadeh, Mohammad Reza Rashidi, Leili Aghebati-Maleki and Mehdi Yousefi

Biosensors and Bioelectronics, 2019, 129, 164
DOI: 10.1016/j.bios.2019.01.008

Ultrasensitive electrochemical Ochratoxin A aptasensor based on CdTe quantum dots functionalized graphene/Au nanocomposites and magnetic separation

Nan Hao, Ling Jiang, Jing Qian and Kun Wang

Journal of Electroanalytical Chemistry, 2016, 781, 332
DOI: 10.1016/j.jelechem.2016.09.053

A lateral flow biosensor based on duplex-specific nuclease and novel isothermal amplification for detection of influenza virus

Xiaoying Zhang, Qianyu Zhou, Shengjun Bu, Yuqi Yang, Lingling Wang, Chuanna Xu, Zhuo Hao, Guijuan Qu and Jiayu Wan

Enzyme and Microbial Technology, 2025, 188, 110653
DOI: 10.1016/j.enzmictec.2025.110653

A reagentless triplex DNA junctions-based electrochemical DNA sensor using signal amplification strategy of CHA and tetraferrocene

Mei Wang, Hanfeng Cui, Nian Hong, Qingxia Shu, Xinru Wang, Yuping Hu, Guobing Wei, Hao Fan and Jing Zhang

Sensors and Actuators B: Chemical, 2022, 358, 131496
DOI: 10.1016/j.snb.2022.131496

Dual-amplified strategy for ultrasensitive electrochemical biosensor based on click chemistry-mediated enzyme-assisted target recycling and functionalized fullerene nanoparticles in the detection of microRNA-141

Lili Zhou, Ting Wang, Yan Bai, Yi Li, Juhui Qiu, Wen Yu and Sheng Zhang

Biosensors and Bioelectronics, 2020, 150, 111964
DOI: 10.1016/j.bios.2019.111964

Ratiometric Electrochemiluminescent/Electrochemical Strategy for Sensitive Detection of MicroRNA Based on Duplex-Specific Nuclease and Multilayer Circuit of Catalytic Hairpin Assembly

Liping Zhu, Mengqian Zhang, Jing Ye, Mengxia Yan, Qiuju Zhu, Jianshe Huang and Xiurong Yang

Anal. Chem., 2020, 92, 8614
DOI: 10.1021/acs.analchem.0c01949

Electrochemical selective detection of carnitine enantiomers coupling copper ion dependent DNAzyme with DNA assistant hybridization chain reaction

Fenfen Zhai, Qiao Yu, Hong Zhou, Jing Liu, Wenrong Yang and Jinmao You

Journal of Electroanalytical Chemistry, 2019, 837, 137
DOI: 10.1016/j.jelechem.2019.02.020

Dual-signal-amplified electrochemiluminescence biosensor for microRNA detection by coupling cyclic enzyme with CdTe QDs aggregate as luminophor

Hong-Yun Zhu and Shou-Nian Ding

Biosensors and Bioelectronics, 2019, 134, 109
DOI: 10.1016/j.bios.2019.04.005

A dual-targeting nanobiosensor for Gender Determination applying Signal Amplification Methods and integrating Fluorometric Gold and Silver Nanoclusters

Fatima Bazzi, Morteza Hosseini, Bahman Ebrahimi-Hoseinzadeh, Haider A. J. Al Lawati and Mohammad Reza Ganjali

Microchim Acta, 2023, 190
DOI: 10.1007/s00604-023-05947-0

Bioinspired Trans‐Scale Functional Interface for Enhanced Enzymatic Dynamics and Ultrasensitive Detection of microRNA

Lei Ye, Fan Yang, Yuanlin Ding, Haibin Yu, Lin Yuan, Qi Dai, Yujie Sun, Xiaoxue Wu, Yang Xiang and Guo‐Jun Zhang

Adv Funct Materials, 2018, 28
DOI: 10.1002/adfm.201706981