Tissue-derived extracellular vesicles: Research progress from isolation to application

Bo Qin, Xi-min Hu, Zhen-hong Su, Xiao-bo Zeng, Hong-ying Ma and Kun Xiong

Pathology - Research and Practice, 2021, 226, 153604
DOI: 10.1016/j.prp.2021.153604

Development of fish liver PLHC-1 spheroids and its applicability to investigate the toxicity of plastic additives

Tiantian Wang, Judith Desmet, Elisabet Pérez-Albaladejo and Cinta Porte

Ecotoxicology and Environmental Safety, 2023, 259, 115016
DOI: 10.1016/j.ecoenv.2023.115016

A 3D fish liver model for aquatic toxicology: Morphological changes and Cyp1a induction in PLHC-1 microtissues after repeated benzo(a)pyrene exposures

April L. Rodd, Norma J. Messier, Charles A. Vaslet and Agnes B. Kane

Aquatic Toxicology, 2017, 186, 134
DOI: 10.1016/j.aquatox.2017.02.018

17α-Ethynylestradiol and Levonorgestrel Exposure of Rainbow Trout RTL-W1 Cells at 18 °C and 21 °C Mainly Reveals Thermal Tolerance, Absence of Estrogenic Effects, and Progestin-Induced Upregulation of Detoxification Genes

Margarida Vilaça, Célia Lopes, Rosária Seabra and Eduardo Rocha

Genes, 2024, 15, 1189
DOI: 10.3390/genes15091189

Development of a 3D spheroid cell culture system from fish cell lines for in vitro infection studies: Evaluation with Saprolegnia parasitica

Marc N. Faber, Jerry M. Sojan, Marcia Saraiva, Pieter van West and Christopher J. Secombes

Journal of Fish Diseases, 2021, 44, 701
DOI: 10.1111/jfd.13331

Potency and mechanism of p-glycoprotein chemosensitizers in rainbow trout (Oncorhynchus mykiss) hepatocytes

Christina U. Johnston and Christopher J. Kennedy

Fish Physiol Biochem, 2024, 50, 2149
DOI: 10.1007/s10695-024-01376-9

A Review of P-Glycoprotein Function and Regulation in Fish

Christina U. Johnston and Christopher J. Kennedy

Fishes, 2024, 9, 51
DOI: 10.3390/fishes9020051

Development of three-dimensional (3D) spheroid culture system from rainbow trout kidney cell line (RTK) for in vitro production of fish viral pathogen

S. Suryakodi, S. Mithra, A. Nafeez Ahmed, K. Kanimozhi, V. Rajkumar, G. Taju, S. Abdul Majeed and A. S. Sahul Hameed

Aquacult Int, 2024, 32, 5029
DOI: 10.1007/s10499-024-01414-6

Effects of Benzo[k]fluoranthene at Two Temperatures on Viability, Structure, and Detoxification-Related Genes in Rainbow Trout RTL-W1 Cell Spheroids

Telma Esteves, Fernanda Malhão, Eduardo Rocha and Célia Lopes

Toxics, 2025, 13, 302
DOI: 10.3390/toxics13040302

Establishing brown trout primary hepatocyte spheroids as a new alternative experimental model—Testing the effects of 5α-dihydrotestosterone on lipid pathways

Inês L. Pereira, Célia Lopes, Eduardo Rocha and Tânia V. Madureira

Aquatic Toxicology, 2022, 253, 106331
DOI: 10.1016/j.aquatox.2022.106331

Production and application of three-dimensional spheroid culture from Lates calcarifer gill cell line for detecting metal using fluorescent probes

Sivaraj Mithra, Shahabudeen Umma Kulsoom, Seepoo Abdul Majeed, Arni Mujthaba Aatif, Nasar Khan Almas, Rahman Hajira Banu, Sayeeda Kaneez Fathima, Gani Taju, Sakvai Mohammed Safiullah and Azeez Sait Sahul Hameed

Inorganic Chemistry Communications, 2025, 177, 114368
DOI: 10.1016/j.inoche.2025.114368

Liver organoids in domestic animals: an expected promise for metabolic studies

Camille Baquerre, Guillaume Montillet and Bertrand Pain

Vet Res, 2021, 52
DOI: 10.1186/s13567-021-00916-y

Matrix Softness-Mediated 3D Zebrafish Hepatocyte Modulates Response to Endocrine Disrupting Chemicals

Kathryn M. Sullivan, Chang Gyun Park, John D. Ito, Mikhail Kandel, Gabriel Popescu, Young Jun Kim and Hyunjoon Kong

Environ. Sci. Technol., 2020, 54, 13797
DOI: 10.1021/acs.est.0c01988

Isolation and characterization of extracellular vesicle subpopulations from tissues

Rossella Crescitelli, Cecilia Lässer and Jan Lötvall

Nat Protoc, 2021, 16, 1548
DOI: 10.1038/s41596-020-00466-1

Toxicokinetics of perfluorooctane sulfonate in rainbow trout (Oncorhynchus mykiss)

Daniel M. Consoer, Alex D. Hoffman, Patrick N. Fitzsimmons, Patricia A. Kosian and John W. Nichols

Environmental Toxicology and Chemistry, 2015, 35, 717
DOI: 10.1002/etc.3230

Considering aspects of the 3Rs principles within experimental animal biology

Lynne U. Sneddon, Lewis G. Halsey and Nic R. Bury

Journal of Experimental Biology, 2017, 220, 3007
DOI: 10.1242/jeb.147058

In vitro salmonid models as tools for studying microbial-derived immunostimulants and aquaculture relevant salmonids pathogens: Current status and future perspectives

Dominic D. Mensah, Ruth Montero, Byron Morales-Lange, Margareth Øverland and Liv Torunn Mydland

Aquaculture, 2025, 595, 741695
DOI: 10.1016/j.aquaculture.2024.741695

Fish hepatocyte spheroids – A powerful (though underexplored) alternative in vitro model to study hepatotoxicity

Rodrigo F. Alves, Eduardo Rocha and Tânia V. Madureira

Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2022, 262, 109470
DOI: 10.1016/j.cbpc.2022.109470

Establishment and long-term maintenance of primary intestinal epithelial cells cultured from the rainbow trout,Oncorhynchus mykiss

Laura M. Langan, Stewart F. Owen and Awadhesh N. Jha

Biology Open, 2018, 7
DOI: 10.1242/bio.032870

Primary hepatocyte culture from Oreochromis niloticus fish as a tool for environmental toxicology

J. Zablocki da Luz, T. Lima de Souza, A. de Almeida Roque, I. Opuskevitch, F. C. Alves da Silva Ferreira, M. Mela Prodocimo, C. Alberto de Oliveira Ribeiro and F. Filipak Neto

Int. J. Environ. Sci. Technol., 2024, 21, 2517
DOI: 10.1007/s13762-023-05129-8

Cytotoxicity of pharmaceuticals and their mixtures toward scaffold-free 3D spheroid cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes

Päivi Järvinen, Maija Kakko and Tiina Sikanen

European Journal of Pharmaceutical Sciences, 2024, 199, 106817
DOI: 10.1016/j.ejps.2024.106817

Biotransformation of Benzo[a]pyrene by Three Rainbow Trout (Onchorhynchus mykiss) Cell Lines and Extrapolation To Derive a Fish Bioconcentration Factor

Julita Stadnicka-Michalak, Frederik T. Weiss, Melanie Fischer, Katrin Tanneberger and Kristin Schirmer

Environ. Sci. Technol., 2018, 52, 3091
DOI: 10.1021/acs.est.7b04548

Research advances of tissue-derived extracellular vesicles in cancers

Wei Li, Jingyao Zhu, Jiayuan Li, Yiyun Jiang, Jiuai Sun, Yan Xu, Hongzhi Pan, Yan Zhou and Jun Zhu

J Cancer Res Clin Oncol, 2024, 150
DOI: 10.1007/s00432-023-05596-z

Development of three-dimensional (3D) spheroid cultures of the continuous rainbow trout liver cell line RTL-W1

Tobias Lammel, Georgia Tsoukatou, Johanna Jellinek and Joachim Sturve

Ecotoxicology and Environmental Safety, 2019, 167, 250
DOI: 10.1016/j.ecoenv.2018.10.009

Impairment of Hypoxia-Induced CA IX by Beta-Blocker Propranolol—Impact on Progression and Metastatic Potential of Colorectal Cancer Cells

Monika Barathova, Katarina Grossmannova, Petra Belvoncikova, Veronika Kubasova, Veronika Simko, Rudolf Skubla, Lucia Csaderova and Jaromir Pastorek

IJMS, 2020, 21, 8760
DOI: 10.3390/ijms21228760

Unveiling the Effects of Two Polycyclic Aromatic Hydrocarbons and Two Temperatures on the Trout RTL-W1 Cell Line Expression of Detoxification-Related Target Genes

Margarida Vilaça, Telma Esteves, Rosária Seabra, Eduardo Rocha and Célia Lopes

JoX, 2025, 15, 84
DOI: 10.3390/jox15030084

Lipidome and Gene Expression Profiling in Zebrafish Liver Spheroids: A 3D Model for Environmental Toxicology Applications

Tiantian Wang, Miquel Perelló Amorós and Cinta Porte

Environ. Sci. Technol., 2025, 59, 12543
DOI: 10.1021/acs.est.5c02300

Comparative analysis of 3D and 2D in vitro models of the permanent fish liver cell line RTL-W1: Metabolic capabilities and responses to xenobiotics

Vanessa Holgersson, Shelby Joyce, Marianne Brookman-Amissah and Tobias Lammel

Ecotoxicology and Environmental Safety, 2024, 288, 117327
DOI: 10.1016/j.ecoenv.2024.117327

Non-invasive Visualization and Characterization of Bile Canaliculus Formation Using Refractive Index Tomography

Kozo Takeuchi and Osamu Yasuhiko

Biological & Pharmaceutical Bulletin, 2024, 47, 1163
DOI: 10.1248/bpb.b24-00066

Fish Cell Spheroids, a Promising In Vitro Model to Mimic In Vivo Research: A Review

Antonio Gómez-Mercader, Luis Monzón-Atienza, Daniel Montero, Jimena Bravo and Félix Acosta

Cells, 2024, 13, 1818
DOI: 10.3390/cells13211818

Tissue‐derived extracellular vesicles in cancers and non‐cancer diseases: Present and future

Su‐Ran Li, Qi‐Wen Man, Xin Gao, Hao Lin, Jing Wang, Fu‐Chuan Su, Han‐Qi Wang, Lin‐Lin Bu, Bing Liu and Gang Chen

J of Extracellular Vesicle, 2021, 10
DOI: 10.1002/jev2.12175

A Step Forward in the Characterization of Primary Brown Trout Hepatocytic Spheroids as Experimental Models

Rodrigo F. Alves, Célia Lopes, Eduardo Rocha and Tânia V. Madureira

Animals, 2023, 13, 2277
DOI: 10.3390/ani13142277

In vitro metabolism of pesticides and industrial chemicals in fish

Toshiyuki Katagi

J. Pestic. Sci., 2020, 45, 1
DOI: 10.1584/jpestics.D19-074

Spheroid Size Does not Impact Metabolism of the β-blocker Propranolol in 3D Intestinal Fish Model

Laura M. Langan, Stewart F. Owen, Maciej Trznadel, Nicholas J. F. Dodd, Simon K. Jackson, Wendy M. Purcell and Awadhesh N. Jha

Front. Pharmacol., 2018, 9
DOI: 10.3389/fphar.2018.00947

Performance of Three-Dimensional Rainbow Trout (Oncorhynchus mykiss) Hepatocyte Spheroids for Evaluating Biotransformation of Pyrene

Maria Therese Hultman, Katharina Bjarnar Løken, Merete Grung, Malcolm J. Reid and Adam Lillicrap

Environmental Toxicology and Chemistry, 2019, 38, 1738
DOI: 10.1002/etc.4476

Pharmaceutical Metabolism in Fish: Using a 3-D Hepatic In Vitro Model to Assess Clearance

Matthew G. Baron, Kate S. Mintram, Stewart F. Owen, Malcolm J. Hetheridge, A. John Moody, Wendy M. Purcell, Simon K. Jackson, Awadhesh N. Jha and Ilaria Corsi

PLoS ONE, 2017, 12, e0168837
DOI: 10.1371/journal.pone.0168837