Issue 19, 2023

An off–on fluorescent nanoprobe for l-cysteine sensing based on the FRET effect

Abstract

A self-assembled fluorescent nanosensor for the determination of L-cysteine (Cys) was constructed based on the mechanism of fluorescence resonance energy transfer (FRET). In this system, CdTe/ZnS QDs serve as the energy donor while AuNPs serve as the receptor, resulting in the occurrence of FRET with dramatic fluorescence quenching of the QDs (turn off). Once Cys is added, AuNPs can adsorb Cys, leading to the release of the QDs. The process would inhibit the FRET, which contributed to the recovery of fluorescence (turn on) and an off–on fluorescence aptasensor for Cys detection was constructed accordingly. The linear response range of the fluorescence sensor is from 0.8 to 50 μM, and the detection limit is 0.24 μM. The sensor demonstrates great sensitivity and selectivity to Cys. More importantly, the QD-based sensing platform was successfully used for the detection of Cys in milk samples with high precision and accuracy, indicating the potential of the probe in practical applications.

Graphical abstract: An off–on fluorescent nanoprobe for l-cysteine sensing based on the FRET effect

Article information

Article type
Paper
Submitted
12 7 2023
Accepted
16 8 2023
First published
19 8 2023

Analyst, 2023,148, 4762-4767

An off–on fluorescent nanoprobe for L-cysteine sensing based on the FRET effect

C. Shi, Y. Zhao, R. Xu and Y. Ding, Analyst, 2023, 148, 4762 DOI: 10.1039/D3AN01173A

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