Issue 18, 2021

Ionozyme: ionic liquids as solvent and stabilizer for efficient bioactivation of CO2

Abstract

The efficient bioactivation of CO2 provides an alternative new strategy for producing high value chemicals and fuels. Normally, in vitro activation of CO2 can be achieved by using formate dehydrogenase (FDH). However, the CO2 solubility and the activity of commercial FDHCb remain a tough challenge for the efficient bioactivation of CO2. Here, we report a new “ionozyme” strategy created by using ionic liquids (ILs) as a solvent and enzyme stabilizer, resulting in a 142.3-fold increase in CO2 conversion over FDHCb. The ionozyme (FDHPa-[CH][Pro]-[TMGH][PhO]) was first developed by combing a discovered novel FDHPa with an efficient synergistic ionic microenvironment. The remarkable performance of this ionozyme is attributed to forming key intermediates [CH][ProCOO] and [TMGH][PhOCOO], stabilizing the enzyme structure with increased solvation structure, and shortening the distance (3.9 Å) between NADH and CO2 to favor the hydride transfer by facilitating their relative orientation and forming new hydrogen bonds at the active sites. This bioactivation of CO2 by this specific ionozyme represents ideal starting points for the sustainable carbon cycle.

Graphical abstract: Ionozyme: ionic liquids as solvent and stabilizer for efficient bioactivation of CO2

Supplementary files

Article information

Article type
Paper
Submitted
14 7 2021
Accepted
17 8 2021
First published
18 8 2021

Green Chem., 2021,23, 6990-7000

Ionozyme: ionic liquids as solvent and stabilizer for efficient bioactivation of CO2

X. Ji, Y. Xue, Z. Li, Y. Liu, L. Liu, P. K. Busk, L. Lange, Y. Huang and S. Zhang, Green Chem., 2021, 23, 6990 DOI: 10.1039/D1GC02503A

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