Issue 41, 2019

An immunochromatographic strip sensor for sildenafil and its analogues

Abstract

In this study, a new hapten of sildenafil (Sild) was successfully synthesized and a sensitive monoclonal antibody (mAb) against Sild was produced based on this new hapten. The subclass of the mAb was IgG2a, and the half-maximal inhibitory concentration (IC50) of the mAb was 0.53 ng mL−1. Next, an immunochromatographic assay (ICA) was established for detecting Sild and its analogues in functional foods, where the visual detection limit (vLOD) and cut-off values were 0.5 and 20 μg kg−1, respectively. With the aid of a strip scan reader, the ICA can measure Sild with an LOD of 0.7 μg kg−1 and a line range of detection between 1.40 and 13.11 μg kg−1. The whole test process takes only 15 min. Therefore, the ICA provides a useful tool for the on-site detection and rapid initial screening of Sild in functional food samples.

Graphical abstract: An immunochromatographic strip sensor for sildenafil and its analogues

Supplementary files

Article information

Article type
Paper
Submitted
09 2 2019
Accepted
08 3 2019
First published
09 3 2019

J. Mater. Chem. B, 2019,7, 6383-6389

An immunochromatographic strip sensor for sildenafil and its analogues

Z. Wang, X. Wu, L. Liu, L. Xu, H. Kuang and C. Xu, J. Mater. Chem. B, 2019, 7, 6383 DOI: 10.1039/C9TB00280D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements