Issue 18, 2014

Vibrational spectroscopic methods for cytology and cellular research

Abstract

The use of vibrational spectroscopy, FTIR and Raman, for cytology and cellular research has the potential to revolutionise the approach to cellular analysis. Vibrational spectroscopy is non-destructive, simple to operate and provides direct information. Importantly it does not require expensive exogenous labels that may affect the chemistry of the cell under analysis. In addition, the advent of spectroscopic microscopes provides the ability to image cells and acquire spectra with a subcellular resolution. This introductory review focuses on recent developments within this fast paced field and highlights potential for the future use of FTIR and Raman spectroscopy. We particularly focus on the development of live cell research and the new technologies and methodologies that have enabled this.

Graphical abstract: Vibrational spectroscopic methods for cytology and cellular research

Article information

Article type
Critical Review
Submitted
08 4 2014
Accepted
27 6 2014
First published
27 6 2014

Analyst, 2014,139, 4411-4444

Author version available

Vibrational spectroscopic methods for cytology and cellular research

G. Clemens, J. R. Hands, K. M. Dorling and M. J. Baker, Analyst, 2014, 139, 4411 DOI: 10.1039/C4AN00636D

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