Issue 37, 2023

Synthesis of ubiquitinated proteins for biochemical and functional analysis

Abstract

Ubiquitination plays a crucial role in controlling various biological processes such as translation, DNA repair and immune response. Protein degradation for example, is one of the main processes which is controlled by the ubiquitin system and has significant implications on human health. In order to investigate these processes and the roles played by different ubiquitination patterns on biological systems, homogeneously ubiquitinated proteins are needed. Notably, these conjugates that are made enzymatically in cells cannot be easily obtained in large amounts and high homogeneity by employing such strategies. Therefore, chemical and semisynthetic approaches have emerged to prepare different ubiquitinated proteins. In this review, we will present the key synthetic strategies and their applications for the preparation of various ubiquitinated proteins. Furthermore, the use of these precious conjugates in different biochemical and functional studies will be highlighted.

Graphical abstract: Synthesis of ubiquitinated proteins for biochemical and functional analysis

Article information

Article type
Review Article
Submitted
17 7 2023
Accepted
27 8 2023
First published
30 8 2023
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2023,14, 10025-10040

Synthesis of ubiquitinated proteins for biochemical and functional analysis

J. Kriegesmann and A. Brik, Chem. Sci., 2023, 14, 10025 DOI: 10.1039/D3SC03664B

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