Issue 42, 2023

A cellular platform for production of C4 monomers

Abstract

Living organisms carry out a wide range of remarkable functions, including the synthesis of thousands of simple and complex chemical structures for cellular growth and maintenance. The manipulation of this reaction network has allowed for the genetic engineering of cells for targeted chemical synthesis, but it remains challenging to alter the program underlying their fundamental chemical behavior. By taking advantage of the unique ability of living systems to use evolution to find solutions to complex problems, we have achieved yields of up to ∼95% for three C4 commodity chemicals, n-butanol, 1,3-butanediol, and 4-hydroxy-2-butanone. Genomic sequencing of the evolved strains identified pcnB and rpoBC as two gene loci that are able to alter carbon flow by remodeling the transcriptional landscape of the cell, highlighting the potential of synthetic pathways as a tool to identify metabolic control points.

Graphical abstract: A cellular platform for production of C4 monomers

Supplementary files

Article information

Article type
Edge Article
Submitted
31 5 2023
Accepted
21 9 2023
First published
09 10 2023
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2023,14, 11718-11726

A cellular platform for production of C4 monomers

M. A. Davis, V. Y. Yu, B. Fu, M. Wen, E. J. Koleski, J. Silverman, C. A. Berdan, D. K. Nomura and M. C. Y. Chang, Chem. Sci., 2023, 14, 11718 DOI: 10.1039/D3SC02773B

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