Issue 43, 2023

Two-dimensional molybdenum disulfide nanosheets evoke nitric oxide-dependent antibacterial effects

Abstract

Nanomaterials are currently being explored as novel antimicrobial agents. In this study, we first investigated the ability of two-dimensional (2D) molybdenum disulfide (MoS2) nanosheets to trigger neutrophil extracellular traps (NETs) using neutrophil-differentiated HL-60 cells as well as primary human peripheral blood neutrophils. We then addressed whether the MoS2 nanosheets themselves function as antibacterial agents. We found that MoS2 and Na2MoO4 both triggered NETs, as evidenced by the quantification of neutrophil elastase (NE) activity and immunofluorescence staining of extracellular NE, as well as scanning electron microscopy. The release of NETs was found to be nitric oxide (NO)-dependent. We also found that the MoS2 nanosheets but not the soluble salt prompted acellular NO production in the presence of NaNO2. The acellular generation of NO, suggestive of nanozyme properties of the MoS2 nanosheets, was demonstrated by electron paramagnetic resonance analysis. Electrochemical analysis using cyclic voltammetry confirmed the redox transition of the MoS2 nanosheets. Finally, MoS2 nanosheets inhibited the growth of Escherichia coli in the presence of sodium nitrate. Taken together, MoS2 nanosheets triggered cellular effects as well as acellular antibacterial effects, and we provided evidence for nitrite reductase-like properties of MoS2.

Graphical abstract: Two-dimensional molybdenum disulfide nanosheets evoke nitric oxide-dependent antibacterial effects

Supplementary files

Article information

Article type
Paper
Submitted
28 6 2023
Accepted
06 10 2023
First published
10 10 2023
This article is Open Access
Creative Commons BY license

Nanoscale, 2023,15, 17409-17421

Two-dimensional molybdenum disulfide nanosheets evoke nitric oxide-dependent antibacterial effects

G. Peng, V. González, E. Vázquez, J. O. Lundberg and B. Fadeel, Nanoscale, 2023, 15, 17409 DOI: 10.1039/D3NR03120A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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