Issue 3, 2023

Ultra-thin membrane filter with a uniformly arrayed nanopore structure for nanoscale separation of extracellular vesicles without cake formation

Abstract

Extracellular vesicles (EVs) have emerged as vehicles that mediate diverse cell–cell communication. However, in-depth understanding of these vesicles is hampered by a lack of a reliable isolation method to separate different types of EVs with high levels of integrity and purity. Here, we developed a nanoporous and ultra-thin membrane structure (NUTS) that warrants the size-based isolation of EVs without cake formation, minimizing the sample loss during the filtration process. By utilizing the micro-electro-mechanical systems (MEMS) technique, we could also control the pore size in nanoscale. We validated the performance of this membrane to separate EVs according to their size range.

Graphical abstract: Ultra-thin membrane filter with a uniformly arrayed nanopore structure for nanoscale separation of extracellular vesicles without cake formation

Supplementary files

Article information

Article type
Paper
Submitted
11 4 2022
Accepted
11 10 2022
First published
22 11 2022
This article is Open Access
Creative Commons BY-NC license

Nanoscale Adv., 2023,5, 640-649

Ultra-thin membrane filter with a uniformly arrayed nanopore structure for nanoscale separation of extracellular vesicles without cake formation

D. Kim, J. Lee, B. Kim, Y. Shin, J. Park, U. Kim, M. Lee, S. B. Kim and S. Kim, Nanoscale Adv., 2023, 5, 640 DOI: 10.1039/D2NA00227B

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