Issue 1, 2022

Metabolic labelling of cancer cells with glycodendrimers stimulate immune-mediated cytotoxicity

Abstract

The recruitment of antibody naturally present in human blood stream at the surface of cancer cells have been proved a promising immunotherapeutic strategy to fight cancer. Antibody recruiting molecules (ARMs) combining tumor and antibody binding modules have been developed for this purpose, however the formation of the interacting complex with both antibody and cell is difficult to optimize to stimulate immune-mediated cytotoxicity. To circumvent this limitation, we report herein a more direct approach combining cell metabolism of azido-sugar and bio-orthogonal click chemistry to conjugate at the cell glycocalyx structurally well-defined glycodendrimers as antibody binding module (ABM). We demonstrate that this strategy allows not only the recruitment of natural antibody at the surface of isolated cells or solid tumor models but also activate a cytotoxic response with human serum as unique source of immune effectors.

Graphical abstract: Metabolic labelling of cancer cells with glycodendrimers stimulate immune-mediated cytotoxicity

Supplementary files

Article information

Article type
Research Article
Submitted
05 8 2021
Accepted
03 11 2021
First published
09 11 2021
This article is Open Access
Creative Commons BY-NC license

RSC Med. Chem., 2022,13, 72-78

Metabolic labelling of cancer cells with glycodendrimers stimulate immune-mediated cytotoxicity

D. Goyard, P. I. Diriwari and N. Berthet, RSC Med. Chem., 2022, 13, 72 DOI: 10.1039/D1MD00262G

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements