Issue 8, 2022

Labeling and sequencing nucleic acid modifications using bio-orthogonal tools

Abstract

The bio-orthogonal reaction is a type of reaction that can occur within a cell without interfering with the active components of the cell. Bio-orthogonal reaction techniques have been used to label and track the synthesis, metabolism, and interactions of distinct biomacromolecules in cells. Thus, it is a handy tool for analyzing biological macromolecules within cells. Nucleic acid modifications are widely distributed in DNA and RNA in cells and play a critical role in regulating physiological and pathological cellular activities. Utilizing bio-orthogonal tools to study modified bases is a critical and worthwhile research direction. The development of bio-orthogonal reactions focusing on nucleic acid modifications has enabled the mapping of nucleic acid modifications in DNA and RNA. This review discusses the recent advances in bio-orthogonal labeling and sequencing nucleic acid modifications in DNA and RNA.

Graphical abstract: Labeling and sequencing nucleic acid modifications using bio-orthogonal tools

Article information

Article type
Review Article
Submitted
30 3 2022
Accepted
20 6 2022
First published
22 6 2022
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2022,3, 994-1007

Labeling and sequencing nucleic acid modifications using bio-orthogonal tools

H. Liu, Y. Wang and X. Zhou, RSC Chem. Biol., 2022, 3, 994 DOI: 10.1039/D2CB00087C

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