Issue 11, 2021

Framework of the kinetic analysis of O2-dependent oxidative biocatalysts for reaction intensification

Abstract

The application of oxygen-dependent enzymes is limited by the low oxygen solubility, a fact that hinders the full operational exploitation of the enzyme activity. This oxygen limitation also creates a difficulty for understanding the intrinsic enzyme kinetics, a critical aspect for the process implementation of oxidative enzymes. Kinetic analysis of O2-dependent enzymes is a case of ping-pong bi-substrate reaction kinetics but with the added feature of a fixed low concentration of oxygen dissolved in the liquid medium. We propose an analysis framework based on a combination of differential methods (based on initial reaction rates-concentration plots) to analyze the main substrate dependency, while the subsequent integral method (consumption time courses of oxygen dissolved) serves to analyze the oxygen dependency. The methodology is applicable by using the oxygen initially dissolved and only working with liquid suspensions. The analysis was applied to paradigmatic case studies with importance in modern green biooxidations. The modeling framework was validated and applied in scale-up reactions in an instrumented aerated stirred tank reactor.

Graphical abstract: Framework of the kinetic analysis of O2-dependent oxidative biocatalysts for reaction intensification

Supplementary files

Article information

Article type
Paper
Submitted
14 6 2021
Accepted
02 8 2021
First published
02 8 2021

React. Chem. Eng., 2021,6, 2058-2074

Framework of the kinetic analysis of O2-dependent oxidative biocatalysts for reaction intensification

A. Lorente-Arevalo, M. Ladero and J. M. Bolivar, React. Chem. Eng., 2021, 6, 2058 DOI: 10.1039/D1RE00237F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements