Issue 1, 2021

A live-cell assay for the detection of pre-microRNA–protein interactions

Abstract

Recent efforts in genome-wide sequencing and proteomics have revealed the fundamental roles that RNA-binding proteins (RBPs) play in the life cycle and function of coding and non-coding RNAs. While these methodologies provide a systems-level view of the networking of RNA and proteins, approaches to enable the cellular validation of discovered interactions are lacking. Leveraging the power of bioorthogonal chemistry- and split-luciferase-based assay technologies, we have devised a conceptually new assay for the live-cell detection of RNA–protein interactions (RPIs), RNA interaction with Protein-mediated Complementation Assay, or RiPCA. As proof-of-concept, we utilized the interaction of the pre-microRNA, pre-let-7, with its binding partner, Lin28. Using this system, we have demonstrated the selective detection of the pre-let-7-Lin28 RPI in both the cytoplasm and nucleus. Furthermore, we determined that this technology can be used to discern relative affinities for specific sequences as well as of individual RNA binding domains. Thus, RiPCA has the potential to serve as a useful tool in supporting the investigation of cellular RPIs.

Graphical abstract: A live-cell assay for the detection of pre-microRNA–protein interactions

Supplementary files

Article information

Article type
Paper
Submitted
28 4 2020
Accepted
26 11 2020
First published
08 12 2020
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2021,2, 241-247

A live-cell assay for the detection of pre-microRNA–protein interactions

S. L. Rosenblum, D. A. Lorenz and A. L. Garner, RSC Chem. Biol., 2021, 2, 241 DOI: 10.1039/D0CB00055H

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