Issue 12, 2021

User-defined, temporal presentation of bioactive molecules on hydrogel substrates using supramolecular coiled coil complexes

Abstract

The ability to spatiotemporally control the presentation of relevant biomolecules in synthetic culture systems has gained significant attention as researchers strive to recapitulate the endogenous extracellular matrix (ECM) in vitro. With the biochemical composition of the ECM constantly in flux, the development of platforms that allow for user-defined control of bioactivity is desired. Here, we reversibly conjugate bioactive molecules to hydrogel-based substrates through supramolecular coiled coil complexes that form between complementary peptides. Our system employs a thiolated peptide for tethering to hydrogel surfaces (T-peptide) through a spatially-controlled photomediated click reaction. The complementary association peptide (A-peptide), containing the bioactive domain, forms a heterodimeric coiled coil complex with the T-peptide. Addition of a disruptor peptide (D-peptide) engineered specifically to target the A-peptide outcompetes the T-peptide for binding, and removes the A-peptide and the attached bioactive motif from the scaffold. We use this platform to demonstrate spatiotemporal control of biomolecule presentation within hydrogel systems in a repeatable process that can be extended to adhesive motifs for cell culture. NIH 3T3 fibroblasts seeded on hyaluronic acid hydrogels and polyethylene glycol-based fibrous substrates supramolecularly functionalized with an RGD motif demonstrated significant cell spreading over their nonfunctionalized counterparts. Upon displacement of the RGD motif, fibroblasts occupied less area and clustured on the substrates. Taken together, this platform enables facile user-defined incorporation and removal of biomolecules in a repeatable process for controlled presentation of bioactivity in engineered culture systems.

Graphical abstract: User-defined, temporal presentation of bioactive molecules on hydrogel substrates using supramolecular coiled coil complexes

Supplementary files

Article information

Article type
Paper
Submitted
05 1 2021
Accepted
28 4 2021
First published
29 4 2021

Biomater. Sci., 2021,9, 4374-4387

User-defined, temporal presentation of bioactive molecules on hydrogel substrates using supramolecular coiled coil complexes

M. G. Grewal, V. P. Gray, R. A. Letteri and C. B. Highley, Biomater. Sci., 2021, 9, 4374 DOI: 10.1039/D1BM00016K

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