Issue 4, 2021

Raman microscopy for skin evaluation

Abstract

Many dermatological studies have had limited success in revealing skin function because conventional histological methods are known to affect skin components. Recent progress in non-invasive optical imaging has enabled non-invasive visualization of the structure of each skin layer. However, it remains difficult to identify individual skin components. Alternatively, it is possible to obtain molecular vibrational signatures using spontaneous Raman scattering microscopy. Spontaneous Raman scattering microscopy requires long acquisition times and is rarely applied to skin imaging, especially because skin components, such as water and transepidermal agents, undergo relatively rapid changes. Consequently, non-linear Raman microscopies, such as coherent anti-Stokes Raman scattering and stimulated Raman scattering, have gradually been applied to acquire molecular imaging of skin tissue. In this review, the applications of Raman microscopies used to evaluate skin and research trends are presented. The applications of spontaneous Raman microscopy to in vivo human skin evaluation are first demonstrated with typical applications. Finally, the latest application of coherent Raman scattering microscopy to visualize 3D intracellular morphologies in the human epidermis during differentiation is described.

Graphical abstract: Raman microscopy for skin evaluation

Article information

Article type
Tutorial Review
Submitted
14 10 2020
Accepted
12 1 2021
First published
18 1 2021

Analyst, 2021,146, 1142-1150

Raman microscopy for skin evaluation

M. Egawa, Analyst, 2021, 146, 1142 DOI: 10.1039/D0AN02039G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements