Issue 20, 2020

Microfluidic paper device for rapid detection of aflatoxin B1 using an aptamer based colorimetric assay

Abstract

Contamination of milk by mycotoxins is a serious problem worldwide. Herein, we focused on the detection of aflatoxin B1 (AflB1) using a paper microfluidic device fabricated with specific aptamers as biorecognition elements. The fabrication process resulted in the generation of a leak proof microfluidic device where two zones were designed: control and test. Detection is achieved by color change when aflatoxin reacts with an aptamer followed by salt induced aggregation of gold nanoparticles. Specific aptamers for aflatoxin B1 were immobilized successfully onto the surface of gold nanoparticles. Biophysical characterization of the conjugated AuNPs–aptamer was done by UV-vis spectroscopy, DLS (dynamic light scattering), TEM (transmission electron microscopy). Under optimal conditions, the microfluidic device showed an excellent response for aflatoxin B1 detection in the range of 1 pM to 1 μM with a detection limit of up to 10 nM in spiked samples. Disadvantages associated with conventional techniques of ELISA were overcome by this one step detection technique with low operation cost, simple instrumentation, and user-friendly format with no interference due to chromatographic separation. The developed microfluidic paper-based analytical device (μPAD) can provide a tool for on-site detection of food toxins in less than a minute which is the main requirement for both qualitative and quantitative analysis in food safety and environmental monitoring.

Graphical abstract: Microfluidic paper device for rapid detection of aflatoxin B1 using an aptamer based colorimetric assay

Supplementary files

Article information

Article type
Paper
Submitted
03 1 2020
Accepted
12 3 2020
First published
24 3 2020
This article is Open Access
Creative Commons BY license

RSC Adv., 2020,10, 11843-11850

Microfluidic paper device for rapid detection of aflatoxin B1 using an aptamer based colorimetric assay

A. Kasoju, N. S. Shrikrishna, D. Shahdeo, A. A. Khan, A. M. Alanazi and S. Gandhi, RSC Adv., 2020, 10, 11843 DOI: 10.1039/D0RA00062K

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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