Issue 9, 2020

Multiplex surface-enhanced Raman scattering detection of deoxynivalenol and ochratoxin A with a linear polymer affinity agent

Abstract

A linear, methacrylamide polymer affinity agent was explored to capture two mycotoxins, deoxynivalenol (DON) and ochratoxin A (OTA), for multiplex surface-enhanced Raman scattering (SERS) detection. These mycotoxins are naturally occurring small molecules from fungi that can be dangerous at low concentrations. SERS detection was completed for each polymer-toxin complex at concentrations relevant to current safety regulation by the FDA: 1 ppm for DON and 5 ppb for OTA. Visibly distinguishable vibrational modes were observed in the multiplex spectra that were attributed to each mycotoxin individually, thus, not requiring any additional chemometric analysis. Density functional theory (DFT) was used to model DON and OTA to accurately label the vibrational modes in the experimental spectra as well as provide insight on the binding between both targets and the affinity agent. Fully modeled vibrations of these toxins are novel contributions due to OTA never being modeled and there being only a few published vibrational modes of DON. DFT guides empirical observations regarding hydrogen bonding at multiple sites of each mycotoxin target molecule through the amine groups on the polymer, confirming the capabilities of a single polymer affinity agent to facilitate multiplex detection of a class of molecules through less-specific interactions than traditional affinity agents.

Graphical abstract: Multiplex surface-enhanced Raman scattering detection of deoxynivalenol and ochratoxin A with a linear polymer affinity agent

Supplementary files

Article information

Article type
Paper
Submitted
15 8 2020
Accepted
25 10 2020
First published
26 10 2020
This article is Open Access
Creative Commons BY-NC license

Mater. Adv., 2020,1, 3256-3266

Multiplex surface-enhanced Raman scattering detection of deoxynivalenol and ochratoxin A with a linear polymer affinity agent

R. S. Rodriguez, V. M. Szlag, T. M. Reineke and C. L. Haynes, Mater. Adv., 2020, 1, 3256 DOI: 10.1039/D0MA00608D

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements