Issue 8, 2019

No evidence of a protective or cumulative negative effect of UV-B on growth inhibition induced by gamma radiation in Scots pine (Pinus sylvestris) seedlings

Abstract

Exposure to ambient UV-B radiation may prime protective responses towards various stressors in plants, though information about interactive effects of UV-B and gamma radiation is scarce. Here, we aimed to test whether UV-B exposure could prime acclimatisation mechanisms contributing to tolerance to low-moderate gamma radiation levels in Scots pine seedlings, and concurrently whether simultaneous UV-B and gamma exposure may have an additive adverse effect on seedlings that had previously not encountered either of these stressors. Responses to simultaneous UV-B (0.35 W m−2) and gamma radiation (10.2–125 mGy h−1) for 6 days with or without UV-B pre-exposure (0.35 W m−2, 4 days) were studied across various levels of organisation, as compared to effects of either radiation type. In contrast to UV-B, and regardless of UV-B presence, gamma radiation at ≥42.9 mGy h−1 caused increased formation of reactive oxygen species and reduced shoot length, and reduced root length at 125 mGy h−1. In all experiments there was a gamma dose rate-dependent increase in DNA damage at ≥10.8 mGy h−1, generally with additional UV-B-induced damage. Gamma-induced growth inhibition and gamma- and UV-B-induced DNA damage were still visible 44 days post-irradiation, even at 20.7 mGy h−1, probably due to genomic instability, but this was reversed after 8 months. In conclusion, there was no evidence of a protective effect of UV-B on gamma-induced growth inhibition and DNA damage in Scots pine, and no additive adverse effect of gamma and UV-B radiation on growth in spite of the additional UV-B-induced DNA damage.

Graphical abstract: No evidence of a protective or cumulative negative effect of UV-B on growth inhibition induced by gamma radiation in Scots pine (Pinus sylvestris) seedlings

Supplementary files

Article information

Article type
Paper
Submitted
31 10 2018
Accepted
06 7 2019
First published
08 7 2019

Photochem. Photobiol. Sci., 2019,18, 1945-1962

No evidence of a protective or cumulative negative effect of UV-B on growth inhibition induced by gamma radiation in Scots pine (Pinus sylvestris) seedlings

D. Blagojevic, Y. Lee, L. Xie, D. A. Brede, L. Nybakken, O. C. Lind, K. E. Tollefsen, B. Salbu, K. A. Solhaug and J. E. Olsen, Photochem. Photobiol. Sci., 2019, 18, 1945 DOI: 10.1039/C8PP00491A

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