Issue 22, 2018

A high spatiotemporal study of somatic exocytosis with scanning electrochemical microscopy and nanoITIES electrodes

Abstract

Extra-synaptic exocytosis is an essential component of cellular communication. A knowledge gap exists in the exocytosis of the non-redox active transmitter acetylcholine. Using the nano-interface between two immiscible electrolyte solutions and scanning electrochemical microscopy (SECM), a high resolution spatiotemporal study of acetylcholine exocytosis is shown from an individual neuronal soma. The nanoelectrode was positioned ∼140 nm away from the release sites on the soma using an SECM. The quantitative study enables the obtaining of key information related to cellular communication, including extracellular concentration of the neurotransmitter, cellular permeability, Ca2+ dependence on somatic release, vesicle density, number of molecules released and the release dynamics. Measurements were achieved with a high signal to noise ratio of 6–19. The released neurotransmitter with a concentration of 2.7 (±1.0) μM was detected at the nanoelectrodes with radii of 750 nm to 860 nm.

Graphical abstract: A high spatiotemporal study of somatic exocytosis with scanning electrochemical microscopy and nanoITIES electrodes

Supplementary files

Article information

Article type
Edge Article
Submitted
09 3 2018
Accepted
14 5 2018
First published
15 5 2018
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2018,9, 4937-4941

A high spatiotemporal study of somatic exocytosis with scanning electrochemical microscopy and nanoITIES electrodes

Theresa M. Welle, K. Alanis, M. L. Colombo, J. V. Sweedler and M. Shen, Chem. Sci., 2018, 9, 4937 DOI: 10.1039/C8SC01131A

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