Issue 8, 2018

Pillararene-based host–guest recognition facilitated magnetic separation and enrichment of cell membrane proteins

Abstract

Here we report a new strategy to separate and enrich cell membrane proteins by means of pillararene-based host–guest recognition and magnetic solid-phase extraction. Due to the high affinity between a water-soluble per-phosphate pillar[5]arene (WP5-P) and its cationic guest, after coating WP5-P on the surface of Fe3O4 nanoparticles, the modified nanoparticles exhibited both magnetic responsiveness and host–guest recognition ability. Furthermore, the WP5-P modified magnetic nanoparticles were successfully applied in separating and enriching guest-tagged cell membrane proteins by means of an external magnetic field from a cell lysate.

Graphical abstract: Pillararene-based host–guest recognition facilitated magnetic separation and enrichment of cell membrane proteins

Supplementary files

Article information

Article type
Research Article
Submitted
01 4 2018
Accepted
24 5 2018
First published
24 5 2018

Mater. Chem. Front., 2018,2, 1475-1480

Pillararene-based host–guest recognition facilitated magnetic separation and enrichment of cell membrane proteins

H. Zhu, J. Liu, B. Shi, H. Wang, Z. Mao, T. Shan and F. Huang, Mater. Chem. Front., 2018, 2, 1475 DOI: 10.1039/C8QM00141C

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