Issue 69, 2018

Incorporation of STED technique into single-molecule spectroscopy to break the concentration limit of diffusing molecules in single-molecule detection

Abstract

By incorporating STED (stimulated emission depletion) nanoscopy into single-molecule spectroscopy, we demonstrate that the concentration limit imposed by optical diffraction can be overcome in diffusion-based single-molecule measurement. We showed that single-molecule detection is feasible at a concentration of 5 nM, which is 100-times higher than the limit of conventional single-molecule measurements.

Graphical abstract: Incorporation of STED technique into single-molecule spectroscopy to break the concentration limit of diffusing molecules in single-molecule detection

Supplementary files

Article information

Article type
Communication
Submitted
15 7 2018
Accepted
02 8 2018
First published
13 8 2018

Chem. Commun., 2018,54, 9667-9670

Incorporation of STED technique into single-molecule spectroscopy to break the concentration limit of diffusing molecules in single-molecule detection

N. Kim, J. Kwon, Y. Lim, J. Kang, S. Bae and S. K. Kim, Chem. Commun., 2018, 54, 9667 DOI: 10.1039/C8CC05726E

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