Issue 1, 2016

Light-driven biocatalytic reduction of α,β-unsaturated compounds by ene reductases employing transition metal complexes as photosensitizers

Abstract

Efficient and cost effective nicotinamide cofactor regeneration is essential for industrial-scale bio-hydrogenations employing flavin-containing biocatalysts such as the Old Yellow Enzymes. A direct flavin regeneration system using visible light to initiate a photoredox cycle and drive biocatalysis is described, and shown to be effective in driving biocatalytic activated alkene reduction. Using Ru(II) or Ir(III) complexes as photosensitizers, coupled with an electron transfer mediator (methyl viologen) and sacrificial electron donor (triethanolamine) drives catalytic turnover of two Old Yellow Enzymes with multiple oxidative substrates. Therefore, there is great potential in the development of light-driven biocatalytic systems, providing an alternative to the reliance on enzyme-based cofactor regeneration systems.

Graphical abstract: Light-driven biocatalytic reduction of α,β-unsaturated compounds by ene reductases employing transition metal complexes as photosensitizers

Supplementary files

Article information

Article type
Paper
Submitted
27 9 2015
Accepted
16 10 2015
First published
21 10 2015
This article is Open Access
Creative Commons BY license

Catal. Sci. Technol., 2016,6, 169-177

Author version available

Light-driven biocatalytic reduction of α,β-unsaturated compounds by ene reductases employing transition metal complexes as photosensitizers

M. K. Peers, H. S. Toogood, D. J. Heyes, D. Mansell, B. J. Coe and N. S. Scrutton, Catal. Sci. Technol., 2016, 6, 169 DOI: 10.1039/C5CY01642H

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