Issue 12, 2014

A TiO2-Au–polymer hybrid system for the photoelectrochemical immunoassay of SirT1

Abstract

Human sirtuin1 (SirT1), which is a member of the sirtuin family, plays an important role in a wide range of cellular processes. Here we demonstrate a new strategy for the photoelectrochemical assay of SirT1 in different cell lines based on a semiconductor–polymer hybrid system consisting of Au–polymer and TiO2-Au nanocomposites. Au–polymer (GC-HBAP) hybrids were synthesized from crosslinked hyperbranched azo-polymer and gold colloids and then used as an immobilization platform for SirT1 antibody. Gold-doped TiO2 (TiO2-Au) nanocomposites were prepared as the photoelectrochemical labels for signal readout in the sandwiched immunoassay. The integration of GC-HBAP with TiO2-Au facilitated the electron transfer and the photoelectrocatalytic reaction, resulting in good analytical performance with high sensitivity, selectivity and rapid response for the analysis of SirT1 levels in different cell lines. This proposed semiconductor–polymer system might open a new perspective for the development of a highly sensitive photoelectrochemical immunosensor, and have potentially promising applications in assays of other proteins.

Graphical abstract: A TiO2-Au–polymer hybrid system for the photoelectrochemical immunoassay of SirT1

Supplementary files

Article information

Article type
Paper
Submitted
19 10 2013
Accepted
16 12 2013
First published
19 12 2013

J. Mater. Chem. B, 2014,2, 1644-1652

A TiO2-Au–polymer hybrid system for the photoelectrochemical immunoassay of SirT1

Y. An, Y. Fu, D. Lu, Y. Wang, W. Bi, Z. Xu, S. Dong, S. Zhang, C. Wang and W. Zhang, J. Mater. Chem. B, 2014, 2, 1644 DOI: 10.1039/C3TB21471K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements