Volume 218, 2019

Focusing on “the important” through targeted NMR experiments: an example of selective 13C–12C bond detection in complex mixtures

Abstract

Current research is attempting to address more complex questions than ever before. As such, the need to follow complex processes in intact media and mixtures is becoming commonplace. Here, a targeted NMR experiment is introduced which selectively detects the formation of 13C–12C bonds in mixtures. This study introduces the experiment on simple standards, and then demonstrates the potential on increasingly complex processes including: fermentation, Arabidopsis thaliana germination/early growth, and metabolism in Daphnia magna both ex vivo and in vivo. As signals from the intact 12C and 13C pools are themselves filtered out, correlations are only observed when a component from each pool combines (i.e. new 13C–12C bonds) in the formation of new structures. This targeted approach significantly reduces the complexity of the mixtures and provides information on the fate and reactivity of carbon in environmental and biological processes. The experiment has application to follow bond formation wherever two pools of carbon are brought together, be it the incorporation of 13C enriched food into a living organism’s biomass, or the degradation of 13C enriched plant material in soil.

Graphical abstract: Focusing on “the important” through targeted NMR experiments: an example of selective 13C–12C bond detection in complex mixtures

Associated articles

Article information

Article type
Paper
Submitted
29 11 2018
Accepted
05 12 2018
First published
05 12 2018

Faraday Discuss., 2019,218, 372-394

Focusing on “the important” through targeted NMR experiments: an example of selective 13C–12C bond detection in complex mixtures

A. Jenne, R. Soong, W. Bermel, N. Sharma, A. Masi, M. Tabatabaei Anaraki and A. Simpson, Faraday Discuss., 2019, 218, 372 DOI: 10.1039/C8FD00213D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements