Issue 11, 2015

Development of an on-animal separation-based sensor for monitoring drug metabolism in freely roaming sheep

Abstract

The development of an on-animal separation-based sensor that can be employed for monitoring drug metabolism in a freely roaming sheep is described. The system consists of microdialysis sampling coupled to microchip electrophoresis with electrochemical detection (MD-ME-EC). Separations were accomplished using an all-glass chip with integrated platinum working and reference electrodes. Discrete samples from the microdialysis flow were introduced into the electrophoresis chip using a flow-gated injection approach. Electrochemical detection was accomplished in-channel using a two-electrode isolated potentiostat. Nitrite was separated by microchip electrophoresis using reverse polarity and a run buffer consisting of 50 mM phosphate at pH 7.4. The entire system was under telemetry control. The system was first tested with rats to monitor the production of nitrite following perfusion of nitroglycerin into the subdermal tissue using a linear probe. The data acquired using the on-line MD-ME-EC system were compared to those obtained by off-line analysis using liquid chromatography with electrochemical detection (LC-EC), using a second microdialysis probe implanted parallel to the first probe in the same animal. The MD-ME-EC device was then used on-animal to monitor the subdermal metabolism of nitroglycerin in sheep. The ultimate goal is to use this device to simultaneously monitor drug metabolism and behavior in a freely roaming animal.

Graphical abstract: Development of an on-animal separation-based sensor for monitoring drug metabolism in freely roaming sheep

Supplementary files

Article information

Article type
Paper
Submitted
22 10 2014
Accepted
05 2 2015
First published
06 2 2015

Analyst, 2015,140, 3820-3829

Author version available

Development of an on-animal separation-based sensor for monitoring drug metabolism in freely roaming sheep

D. E. Scott, S. D. Willis, S. Gabbert, D. Johnson, E. Naylor, E. M. Janle, J. E. Krichevsky, C. E. Lunte and S. M. Lunte, Analyst, 2015, 140, 3820 DOI: 10.1039/C4AN01928H

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